This study explored the value priorities of the post-80s generation in China major cities and its impact to the individuals' subjective well-being (SWB). Data were collected using questionnaires about values and SWB. Samples of 249 adults who were born in 1980s from Beijing, Shanghai, Guangzhou, Shenzhen participated in online questionnaire survey. The results show that: (1) Top to down, value priorities are: family, security, harmoniousness, virtue, freedom, enjoyment, relationship, tradition, success and innovation. (2) Virtue, family and relationship are positively correlated with SWB. Success and enjoyment are negatively correlated with SWB. (3) The SWB of feminine values dominated group is higher than masculine values dominated group (feminine values: harmoniousness, virtue, tradition, security, family and freedom; masculine values: success, innovation, relationship and enjoyment). The implications of these findings for research and practice are discussed.