Does capillary racetrack-based enrichment reflect the diversity of uncultivated magnetotactic cocci in environmental samples?
文献类型：期刊论文
| 作者 | Lin, Wei; Tian, Lanxiang; Li, Jinhua; Pan, Yongxin |
| 刊名 | FEMS MICROBIOLOGY LETTERS
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 279期号:2页码:202-206 |
| 关键词 | phylogenetic diversity 16S rRNA gene capillary racetrack enrichment metagenome-based PCR magnetotactic cocci |
| ISSN号 | 0378-1097 |
| DOI | 10.1111/j.1574-6968.2007.01029.x |
| 文献子类 | Article |
| 英文摘要 | The racetrack-based PCR approach is widely used in phylogenetic analysis of magnetotactic bacteria (MTB), which are isolated from environmental samples using the capillary racetrack method. To evaluate whether the capillary racetrack-based enrichment can truly reflect the diversity of MTB in the targeted environmental sample, phylogenetic diversity studies of MTB enriched from the Miyun lake near Beijing were carried out, using both the capillary racetrack-based PCR and a modified metagenome-based PCR approach. Magnetotactic cocci were identified in the studied sample using both approaches. Comparative studies showed that three clusters of magnetotactic cocci were revealed by the modified metagenome-based PCR approach, while only one of them (e.g. MYG-22 sequence) was detected by the racetrack-based PCR approach from the studied sample. This suggests that the result of capillary racetrack-based enrichment might have been biased by the magnetotaxis of magnetotactic bacteria. It appears that the metagenome-based PCR approach better reflects the original diversity of MTB in the environmental sample. |
| WOS关键词 | ELECTRON-MICROSCOPY ; SEQUENCE ALIGNMENT ; BACTERIA ; DNA ; MAGNETOSOMES ; SEDIMENT ; FE3S4 ; FE3O4 |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000252557300010 |
| 出版者 | BLACKWELL PUBLISHING |
| 源URL | [http://ir.iggcas.ac.cn/handle/132A11/70172]  |
| 专题 | 中国科学院地质与地球物理研究所 |
| 通讯作者 | Pan, Yongxin |
| 作者单位 | Chinese Acad Sci, Inst Geol & Geophys, State Key Lab Lithospher Evolut, Paleomagnetism & Geochronol Lab,Biogeomagnetism G, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Wei,Tian, Lanxiang,Li, Jinhua,et al. Does capillary racetrack-based enrichment reflect the diversity of uncultivated magnetotactic cocci in environmental samples?[J]. FEMS MICROBIOLOGY LETTERS,2008,279(2):202-206. |
| APA | Lin, Wei,Tian, Lanxiang,Li, Jinhua,&Pan, Yongxin.(2008).Does capillary racetrack-based enrichment reflect the diversity of uncultivated magnetotactic cocci in environmental samples?.FEMS MICROBIOLOGY LETTERS,279(2),202-206. |
| MLA | Lin, Wei,et al."Does capillary racetrack-based enrichment reflect the diversity of uncultivated magnetotactic cocci in environmental samples?".FEMS MICROBIOLOGY LETTERS 279.2(2008):202-206. |
入库方式： OAI收割
来源：地质与地球物理研究所
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