Diversity analysis of magnetotactic bacteria in Lake Miyun, northern China, by restriction fragment length polymorphism
文献类型:期刊论文
作者 | Lin, Wei1,2; Li, Jinhua1,2; Schueler, Dirk; Jogler, Christian3; Pan, Yongxin1 |
刊名 | SYSTEMATIC AND APPLIED MICROBIOLOGY
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出版日期 | 2009-08-01 |
卷号 | 32期号:5页码:342-350 |
关键词 | Magnetotactic bacteria Restriction fragment length polymorphism Phylogenetic analysis 16S rRNA Transmission electron microscopy |
ISSN号 | 0723-2020 |
DOI | 10.1016/j.syapm.2008.10.005 |
文献子类 | Article |
英文摘要 | Magnetotactic bacteria (MTB) synthesize intracellular nano-scale crystals of magnetite or greigite within magnetosomes. MTB are ubiquitous in limnic and marine environments. In order to understand the diversity of MTB better, sediment samples were examined from Lake Miyun near Beijing by restriction fragment length polymorphism (RFLP). First, in silico analysis was used to evaluate the effectiveness of 12 sets of restriction endonucleases for distinguishing MTB sequences retrieved from the GenBank database. It was found that the tested restriction endonucleases had different power in the ability to differentiate the operational taxonomic units (OTUs) of MTB. Specifically, of the 12 sets of enzymes, MspI plus Rsal was found to be the most effective for correctly differentiating the OTUs of selected MTB sequences and it could detect 16 OTUs with appropriate OTUmin and OTUmax values (96.7% and 97.7%, respectively). The MspI plus Rsal RFLP analysis was then utilized to investigate the diversity of MTB in Lake Miyun sediment and it identified 8 OTUs (74.5% of the whole library) as MTB. Among these, 5 were affiliated to Alphaproteobacteria, while the rest belonged to the Nitrospira phylum. Interestingly, OTUs C, D and I displayed 91.8-98.4% similarity to "Magnetobacterium bavaricum". Together, these results demonstrated that the MspI plus RyaI RFLP analysis was useful for studying the diversity and change in community composition of uncultivated MTB from environmental samples. (C) 2008 Elsevier GmbH. AD rights reserved. |
WOS关键词 | RIBOSOMAL-RNA GENES ; HYDROTHERMAL VENT SYSTEM ; FRESH-WATER SEDIMENT ; MICROBIAL DIVERSITY ; PHYLOGENETIC DIVERSITY ; ELECTRON-MICROSCOPY ; MARINE-SEDIMENTS ; LOIHI SEAMOUNT ; SALT POND ; DEEP-SEA |
WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000269071200007 |
出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
资助机构 | CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; 40325011) ; 40325011) ; 40325011) ; 40325011) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; 40325011) ; 40325011) ; 40325011) ; 40325011) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; 40325011) ; 40325011) ; 40325011) ; 40325011) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; 40325011) ; 40325011) ; 40325011) ; 40325011) |
源URL | [http://ir.iggcas.ac.cn/handle/132A11/70758] ![]() |
专题 | 中国科学院地质与地球物理研究所 |
通讯作者 | Pan, Yongxin |
作者单位 | 1.Chinese Acad Sci, Inst Geol & Geophys, State Key Lab Lithospher Evolut, Biogeomagnetism Grp,Paleomagnetism & Geochronol L, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Univ Munich, Dept Biol 1, Bereich Mikrobiol, D-80638 Munich, Germany |
推荐引用方式 GB/T 7714 | Lin, Wei,Li, Jinhua,Schueler, Dirk,et al. Diversity analysis of magnetotactic bacteria in Lake Miyun, northern China, by restriction fragment length polymorphism[J]. SYSTEMATIC AND APPLIED MICROBIOLOGY,2009,32(5):342-350. |
APA | Lin, Wei,Li, Jinhua,Schueler, Dirk,Jogler, Christian,&Pan, Yongxin.(2009).Diversity analysis of magnetotactic bacteria in Lake Miyun, northern China, by restriction fragment length polymorphism.SYSTEMATIC AND APPLIED MICROBIOLOGY,32(5),342-350. |
MLA | Lin, Wei,et al."Diversity analysis of magnetotactic bacteria in Lake Miyun, northern China, by restriction fragment length polymorphism".SYSTEMATIC AND APPLIED MICROBIOLOGY 32.5(2009):342-350. |
入库方式: OAI收割
来源:地质与地球物理研究所
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