中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Diversity analysis of magnetotactic bacteria in Lake Miyun, northern China, by restriction fragment length polymorphism

文献类型:期刊论文

作者Lin, Wei1,2; Li, Jinhua1,2; Schueler, Dirk; Jogler, Christian3; Pan, Yongxin1
刊名SYSTEMATIC AND APPLIED MICROBIOLOGY
出版日期2009-08-01
卷号32期号:5页码:342-350
关键词Magnetotactic bacteria Restriction fragment length polymorphism Phylogenetic analysis 16S rRNA Transmission electron microscopy
ISSN号0723-2020
DOI10.1016/j.syapm.2008.10.005
文献子类Article
英文摘要Magnetotactic bacteria (MTB) synthesize intracellular nano-scale crystals of magnetite or greigite within magnetosomes. MTB are ubiquitous in limnic and marine environments. In order to understand the diversity of MTB better, sediment samples were examined from Lake Miyun near Beijing by restriction fragment length polymorphism (RFLP). First, in silico analysis was used to evaluate the effectiveness of 12 sets of restriction endonucleases for distinguishing MTB sequences retrieved from the GenBank database. It was found that the tested restriction endonucleases had different power in the ability to differentiate the operational taxonomic units (OTUs) of MTB. Specifically, of the 12 sets of enzymes, MspI plus Rsal was found to be the most effective for correctly differentiating the OTUs of selected MTB sequences and it could detect 16 OTUs with appropriate OTUmin and OTUmax values (96.7% and 97.7%, respectively). The MspI plus Rsal RFLP analysis was then utilized to investigate the diversity of MTB in Lake Miyun sediment and it identified 8 OTUs (74.5% of the whole library) as MTB. Among these, 5 were affiliated to Alphaproteobacteria, while the rest belonged to the Nitrospira phylum. Interestingly, OTUs C, D and I displayed 91.8-98.4% similarity to "Magnetobacterium bavaricum". Together, these results demonstrated that the MspI plus RyaI RFLP analysis was useful for studying the diversity and change in community composition of uncultivated MTB from environmental samples. (C) 2008 Elsevier GmbH. AD rights reserved.
WOS关键词RIBOSOMAL-RNA GENES ; HYDROTHERMAL VENT SYSTEM ; FRESH-WATER SEDIMENT ; MICROBIAL DIVERSITY ; PHYLOGENETIC DIVERSITY ; ELECTRON-MICROSCOPY ; MARINE-SEDIMENTS ; LOIHI SEAMOUNT ; SALT POND ; DEEP-SEA
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
语种英语
WOS记录号WOS:000269071200007
出版者ELSEVIER GMBH, URBAN & FISCHER VERLAG
资助机构CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; 40325011) ; 40325011) ; 40325011) ; 40325011) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; 40325011) ; 40325011) ; 40325011) ; 40325011) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; 40325011) ; 40325011) ; 40325011) ; 40325011) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; CAS(KZCX-3-SW-150) ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; NSFC(40821091 ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; Max Planck/CAS doctoral promotion program (DPP) ; 40325011) ; 40325011) ; 40325011) ; 40325011)
源URL[http://ir.iggcas.ac.cn/handle/132A11/70758]  
专题中国科学院地质与地球物理研究所
通讯作者Pan, Yongxin
作者单位1.Chinese Acad Sci, Inst Geol & Geophys, State Key Lab Lithospher Evolut, Biogeomagnetism Grp,Paleomagnetism & Geochronol L, Beijing 100029, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Univ Munich, Dept Biol 1, Bereich Mikrobiol, D-80638 Munich, Germany
推荐引用方式
GB/T 7714
Lin, Wei,Li, Jinhua,Schueler, Dirk,et al. Diversity analysis of magnetotactic bacteria in Lake Miyun, northern China, by restriction fragment length polymorphism[J]. SYSTEMATIC AND APPLIED MICROBIOLOGY,2009,32(5):342-350.
APA Lin, Wei,Li, Jinhua,Schueler, Dirk,Jogler, Christian,&Pan, Yongxin.(2009).Diversity analysis of magnetotactic bacteria in Lake Miyun, northern China, by restriction fragment length polymorphism.SYSTEMATIC AND APPLIED MICROBIOLOGY,32(5),342-350.
MLA Lin, Wei,et al."Diversity analysis of magnetotactic bacteria in Lake Miyun, northern China, by restriction fragment length polymorphism".SYSTEMATIC AND APPLIED MICROBIOLOGY 32.5(2009):342-350.

入库方式: OAI收割

来源:地质与地球物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。