Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments
文献类型：期刊论文
| 作者 | Jogler, Christian1; Lin, Wei2; Meyerdierks, Anke3; Kube, Michael4; Katzmann, Emanuel1; Flies, Christine3; Pan, Yongxin2; Amann, Rudolf3; Reinhardt, Richard4; Schueler, Dirk1 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2009-06-15 |
| 卷号 | 75期号:12页码:3972-3979 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/AEM.02701-08 |
| 文献子类 | Article |
| 英文摘要 | In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fosmid libraries were constructed and screened by end sequencing and hybridization analysis using heterologous magnetosome gene probes. A total of 14 fosmids were fully sequenced. We identified and characterized two fosmids, most likely originating from two different alphaproteobacterial strains of MTB that contain several putative operons with homology to the magnetosome island (MAI) of cultivated MTB. This is the first evidence that uncultivated MTB exhibit similar yet differing organizations of the MAI, which may account for the diversity in biomineralization and magnetotaxis observed in MTB from various environments. |
| WOS关键词 | FRESH-WATER SEDIMENT ; MAGNETIC BACTERIA ; NATURAL-PRODUCTS ; DIVERSITY ; REVEALS ; GENOMES ; ACCESS ; MICROORGANISMS ; LIBRARIES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000266782300018 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 资助机构 | German DFG ; German DFG ; BMBF ; BMBF ; MPG ; MPG ; German DFG ; German DFG ; BMBF ; BMBF ; MPG ; MPG ; German DFG ; German DFG ; BMBF ; BMBF ; MPG ; MPG ; German DFG ; German DFG ; BMBF ; BMBF ; MPG ; MPG |
| 源URL | [http://ir.iggcas.ac.cn/handle/132A11/70862]  |
| 专题 | 中国科学院地质与地球物理研究所 |
| 通讯作者 | Schueler, Dirk |
| 作者单位 | 1.LMU Dept Biol I, D-82152 Planegg Martinsried, Germany 2.Chinese Acad Sci, State Key Lab Lithospher Evolut, Paleomagnetism & Geochronol Lab, Biogeomagnetism Grp,Inst Geol & Geophys, Beijing 100029, Peoples R China 3.Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany 4.Max Planck Inst Mol Genet, D-14195 Berlin, Germany |
| 推荐引用方式 GB/T 7714 | Jogler, Christian,Lin, Wei,Meyerdierks, Anke,et al. Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2009,75(12):3972-3979. |
| APA | Jogler, Christian.,Lin, Wei.,Meyerdierks, Anke.,Kube, Michael.,Katzmann, Emanuel.,...&Schueler, Dirk.(2009).Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,75(12),3972-3979. |
| MLA | Jogler, Christian,et al."Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75.12(2009):3972-3979. |
入库方式： OAI收割
来源：地质与地球物理研究所
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