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Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation

文献类型：期刊论文

作者Li Jinhua1,2; Ge Xin1; Zhang Xiaokui3; Chen Guanjun1; Pan Yongxin2
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2010-07-01
卷号28期号:4页码:826-831
关键词magnetotactic bacteria Magnetospirillum magneticum AMB-1 capillary magnetic separation strain recovery
ISSN号0254-4059
DOI10.1007/s00343-010-9068-4
文献子类Article
英文摘要Cultivable magnetotactic bacteria (MTB) in laboratory can provide sufficient samples for molecular microbiological and magnetic studies. However, a cold-stored MTB strain, such as Magnetospirillum magneticum AMB-1, often loses its ability to synthesize magnetosomes and consequently fails to sense the external magnetic field. It is therefore important to quickly recover vigorous bacteria cells that highly capable of magnetosome producing. In this study, a modified capillary magnetic separation system was designed to recover a deteriorating strain of Magnetospirillum magneticum AMB-1 that long-term cold-stored in a refrigerator. The results show that all cells obtained after a 3-cycle treatment were vigorous and had the ability to produce magnetosomes. Moreover, the 3(rd)-cycle recovered cells were able to form more magnetosome crystals. Compared with the colony formation method, this new method is time-saving, easily operated, and more efficient for recovering vigorous MTB cells.
WOS关键词MAGNETOTACTIC BACTERIA ; MAGNETOSOME ; GRYPHISWALDENSE ; ISLAND ; COCCI
WOS研究方向Marine & Freshwater Biology ; Oceanography
语种英语
WOS记录号WOS:000279294400016
出版者SCIENCE PRESS
资助机构Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011)
源URL[http://ir.iggcas.ac.cn/handle/132A11/72486]  
专题中国科学院地质与地球物理研究所
通讯作者Chen Guanjun
作者单位1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China
2.Chinese Acad Sci, Biogeomagnetism Grp, Paleomagnetism & Geochronol Lab SKL LE, Inst Geol & Geophys, Beijing 100029, Peoples R China
3.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China
推荐引用方式
GB/T 7714		 Li Jinhua,Ge Xin,Zhang Xiaokui,et al. Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(4):826-831.
APA Li Jinhua,Ge Xin,Zhang Xiaokui,Chen Guanjun,&Pan Yongxin.(2010).Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(4),826-831.
MLA Li Jinhua,et al."Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.4(2010):826-831.

入库方式： OAI收割

来源：地质与地球物理研究所

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