Construction of a microrobot system using magnetotactic bacteria for the separation of Staphylococcus aureus
文献类型：期刊论文
| 作者 | Chen, Chang-You1,2,3; Chen, Chuan-Fang1,3; Yi, Yong4; Chen, Lin-Jie1,2,3; Wu, Long-Fei3,5; Song, Tao1,3 |
| 刊名 | BIOMEDICAL MICRODEVICES
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 16期号:5页码:761-770 |
| 关键词 | Magnetotactic bacteria Staphylococcus aureus Microfluidic chip Microrobot Separation |
| ISSN号 | 1387-2176 |
| DOI | 10.1007/s10544-014-9880-2 |
| 文献子类 | Article |
| 英文摘要 | Magnetotactic bacteria exhibit superiority over other bacteria in fabricating microrobots because of their high motility and convenient controllability. In this study, a microrobot system is constructed using magnetotactic bacteria MO-1 and applied in pathogenic separation. The feasibility of this approach is demonstrated using Staphylococcus aureus. The MO-1 magnetotactic bacterial microrobots are fabricated by binding magnetotactic bacteria MO-1 with their rabbit anti-MO-1 polyclonal antibodies. The efficient binding of MO-1 magnetotactic bacterial microrobots to Staphylococcus aureus is corroborated by phase contrast microscopic and transmission electron microscopic analyses. Further, a microfluidic chip is designed and produced, and the MO-1 microrobots are magnetically guided toward a sample pool in the chip. In the sample pool, Staphylococcus aureus samples are loaded on the microrobots and then carried away to a detection pool in the chip, suggesting the microrobots have successfully carried and separated pathogen. This study is the first to demonstrate bacterial microrobots carrying pathogens and more importantly, it reflects the great potential of using magnetotactic bacteria to develop magnetic-guided, auto-propelled microrobots for pathogen isolation. |
| WOS关键词 | IMMUNOMAGNETIC SEPARATION ; MAGNETIC NANOPARTICLES ; MAGNETOSOMES ; MICROBEADS ; DELIVERY ; ASSAY |
| WOS研究方向 | Engineering ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000341658300014 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science of China(51037006) ; National Natural Science of China(51037006) ; National Natural Science of China(51037006) ; National Natural Science of China(51037006) ; National Natural Science of China(51037006) ; National Natural Science of China(51037006) ; National Natural Science of China(51037006) ; National Natural Science of China(51037006) |
| 源URL | [http://ir.iggcas.ac.cn/handle/132A11/85073]  |
| 专题 | 中国科学院地质与地球物理研究所 |
| 通讯作者 | Song, Tao |
| 作者单位 | 1.Chinese Acad Sci, Beijing Key Lab Bioelectromagnetism, Inst Elect Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.France China Biomineralizat & Nanostruct Lab, Beijing 100193, Peoples R China 4.306 Hosp, Beijing 100101, Peoples R China 5.Aix Marseille Univ, CNRS UMR7283, Inst Microbiol Mediterranee, Chim Bacterienne Lab, F-13402 Marseille 20, France |
| 推荐引用方式 GB/T 7714 | Chen, Chang-You,Chen, Chuan-Fang,Yi, Yong,et al. Construction of a microrobot system using magnetotactic bacteria for the separation of Staphylococcus aureus[J]. BIOMEDICAL MICRODEVICES,2014,16(5):761-770. |
| APA | Chen, Chang-You,Chen, Chuan-Fang,Yi, Yong,Chen, Lin-Jie,Wu, Long-Fei,&Song, Tao.(2014).Construction of a microrobot system using magnetotactic bacteria for the separation of Staphylococcus aureus.BIOMEDICAL MICRODEVICES,16(5),761-770. |
| MLA | Chen, Chang-You,et al."Construction of a microrobot system using magnetotactic bacteria for the separation of Staphylococcus aureus".BIOMEDICAL MICRODEVICES 16.5(2014):761-770. |
入库方式： OAI收割
来源：地质与地球物理研究所
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