Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis
文献类型：期刊论文
| 作者 | LU Ting ; Edward P C Lai ; Jorn C C Yu ; HU Fengzu |
| 刊名 | 食品科学
 |
| 出版日期 | 2006 |
| 卷号 | 27期号:12页码:582-587 |
| 关键词 | 分析 黄酮类化合物 蜂花粉 毛细管电泳 |
| 合作状况 | 国内 |
| 中文摘要 | a capillary electrophoresis (ce) method has been developed for the determination of six bioactive flavonoids that are commonly found in health foods: hesperidin, hyperin, isorhamnetin, kaempferol, quercetin and rutin. the effects of several parameters, such as ph, buffer concentration, separation voltage and uv detector wavelength, were investigated to find the optimal conditions. using a hbbch-naib-io? buffer (ph 9.2), the analytes can be separated within 8 min. the relative standard deviations of migration times in eight injections were between 0.77% and 0.93%, and those of the peak areas ranged from 3.8% to 8.6%. a high reproducibility and excellent linearity was observed over two orders of magnitude, with detection limits (s/n = 3) ranging from 0.34ug ml to 2.9ug ml for all the six analytes. recoveries ranged from 80.4 % to 113.9 %. the new method is simple, reproducible and sensitive. no solid phase extraction for sample pretreatment is necessary. analysis results are accurate in application to bee pollens. |
| 英文摘要 | a capillary electrophoresis (ce) method has been developed for the determination of six bioactive flavonoids that are commonly found in health foods: hesperidin, hyperin, isorhamnetin, kaempferol, quercetin and rutin. the effects of several parameters, such as ph, buffer concentration, separation voltage and uv detector wavelength, were investigated to find the optimal conditions. using a hbbch-naib-io? buffer (ph 9.2), the analytes can be separated within 8 min. the relative standard deviations of migration times in eight injections were between 0.77% and 0.93%, and those of the peak areas ranged from 3.8% to 8.6%. a high reproducibility and excellent linearity was observed over two orders of magnitude, with detection limits (s/n = 3) ranging from 0.34ug ml to 2.9ug ml for all the six analytes. recoveries ranged from 80.4 % to 113.9 %. the new method is simple, reproducible and sensitive. no solid phase extraction for sample pretreatment is necessary. analysis results are accurate in application to bee pollens. |
| 学科主题 | 生物科学 |
| 收录类别 | CSCD |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2625250 |
| 公开日期 | 2009-11-16 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/306]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | LU Ting,Edward P C Lai,Jorn C C Yu,et al. Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis[J]. 食品科学,2006,27(12):582-587. |
| APA | LU Ting,Edward P C Lai,Jorn C C Yu,&HU Fengzu.(2006).Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis.食品科学,27(12),582-587. |
| MLA | LU Ting,et al."Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis".食品科学 27.12(2006):582-587. |
入库方式： OAI收割
来源：西北高原生物研究所
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