Three-dimensional light field microscope based on a lenslet array
文献类型：期刊论文
| 作者 | Zhang, Mei ; Geng, Zheng; Pei, Renjing; Cao, Xuan; Zhang, Zhaoxing
|
| 刊名 | OPTICS COMMUNICATIONS
 |
| 出版日期 | 2017-11-15 |
| 期号 | 403页码:133-142 |
| 关键词 | Light Field Three-dimensional Microscopy High Resolution 3d Image Acquisition 3d Image Reconstruction |
| DOI | 10.1016/j.optcom.2017.07.026 |
| 文献子类 | Article |
| 英文摘要 | Most conventional microscopes can only acquire two-dimensional (2D) images. However, there is increasing demand for microscopes capable of acquiring three-dimensional (3D) images for use in biological research. To improve observations of in vivo specimens, we develop a novel one snap-shot 3D light field microscope with high spatial resolution. By adding a lenslet array and focusing optics into the optical train of a conventional microscope, we are able to simultaneously capture seven sub-aperture images from different perspectives in a single shot, and finish 3D surface reconstruction of the specimen. In this paper, we present a functioning prototype of a novel 3D light field microscope using different objectives, and describe its design and image quality. During 3D image reconstruction, we use an optical flow method and back-projection interpolation to improve the resolution of the subimages. The performance of the novel light field microscope is analyzed theoretically and experimentally. Multiple perspective images are obtained using an objective with 20 x /NA 0.4. These images are 1050 x 1050 pixels with a depth of view of 200 mu m. For our microscope, the highest resolvable group of USAF 1951 target is about group 7.5. 3D reconstructions of various specimens are successfully created using these images. This novel microscopy system is suitable for various applications in 3D imaging and optical metrology. (C) 2017 Elsevier B.V. All rights reserved. |
| WOS关键词 | ILLUMINATION MICROSCOPY ; NEURONAL-ACTIVITY |
| WOS研究方向 | Optics |
| 语种 | 英语 |
| WOS记录号 | WOS:000410638900021 |
| 资助机构 | 863 Program(2015AA015905) ; National Natural Science Foundation of China (NSFC)(61605240) |
| 源URL | [http://ir.ia.ac.cn/handle/173211/20728]  |
| 专题 | 自动化研究所_复杂系统管理与控制国家重点实验室_先进控制与自动化团队 |
| 作者单位 | Chinese Acad Sci, Inst Automat, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Mei,Geng, Zheng,Pei, Renjing,et al. Three-dimensional light field microscope based on a lenslet array[J]. OPTICS COMMUNICATIONS,2017(403):133-142. |
| APA | Zhang, Mei,Geng, Zheng,Pei, Renjing,Cao, Xuan,&Zhang, Zhaoxing.(2017).Three-dimensional light field microscope based on a lenslet array.OPTICS COMMUNICATIONS(403),133-142. |
| MLA | Zhang, Mei,et al."Three-dimensional light field microscope based on a lenslet array".OPTICS COMMUNICATIONS .403(2017):133-142. |
入库方式： OAI收割
来源：自动化研究所
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