Imaging Dendrimer-Grafted Graphene Oxide Mediated Anti-miR-21 Delivery With an Activatable Luciferase Reporter
文献类型：期刊论文
| 作者 | Wang, Fu1; Zhang, Beilei2; Zhou, Lin1; Shi, Yaru1; Li, Zhiqiang1; Xia, Yuqiong1; Tian, Jie1,3
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| 刊名 | ACS APPLIED MATERIALS & INTERFACES
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| 出版日期 | 2016-04-13 |
| 卷号 | 8期号:14页码:9014-9021 |
| 关键词 | Mirna Delivery Graphene Oxide Dendrimer Luciferase Reporter Bioluminescence Imaging |
| DOI | 10.1021/acsami.6b02662 |
| 文献子类 | Article |
| 英文摘要 | MicroRNAs (miRNAs) are a class of post-transcriptional gene regulators involved in various physiological processes including carcinogenesis, and they have emerged as potential targets for tumor theranostics. However, the employment of antisense oligonucleotides, termed anti-miRs, for antagonizing miRNA functions in vivo has largely been impeded by a lack of effective delivery carriers. Here, we describe the development of polyamidoamine (PAMAM) dendrimer and polyethylene glycol (PEG)-functionalized nanographene oxide (NGO) conjugate (NGO-PEG-dendrimer) for the efficient delivery of anti-miR-21 into non-small-cell lung cancer cells. To monitor the delivery of anti-miR-21 into cells and tumors, we also constructed an activatable luciferase reporter (Fluc-3xPS) containing three perfectly complementary sequences against miR-21 in the 3' untranslated region (UTR) of the reporter. Compared with bare dendrimer and Lipofectamine 2000 (Lipo2000), NGO-PEG-dendrimer showed considerably lower cytotoxicity and higher transfection efficiency. As demonstrated by in vitro bioluminescence imaging and Western blotting assays, NGO-PEG-dendrimer effectively delivered anti-miR-21 into the cytoplasm and resulted in the upregulation of luciferase intensity and PTEN target protein expression in a dose-dependent manner. Moreover, transfection with anti-miR-21 by NGO-PEG-dendrimer led to stronger inhibition of cell migration and invasion than did bare dendrimer or Lipo2000 transfection. The intravenous delivery of anti-miR-21 via NGO-PEG-dendrimer induced a significant increase in the bioluminescence signal within the Flue-UPS reporter-transplanted tumor areas. These results suggest that NGO-PEG-dendrimer could be an efficient and a potential nanocarrier for delivering RNA oligonucleotides. In addition, the strategy of combining NGO-PEG-dendrimer with an activatable luciferase reporter allows the image-guided monitoring of the delivery process, which can provide insights into the RNA-based cancer treatments. |
| WOS关键词 | NANO-GRAPHENE ; HUMAN CANCER ; MICRORNAS ; POLYETHYLENIMINE ; GLYCOL ; DRUGS ; CELLS |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000374274900020 |
| 资助机构 | National Natural Science Foundation of China(81301214 ; National Basic Research and Development Program of China (973 Program)(2013CB733803) ; 81571721) |
| 源URL | [http://ir.ia.ac.cn/handle/173211/12213]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 通讯作者 | Wang, Fu |
| 作者单位 | 1.Xidian Univ, Sch Life Sci & Technol, Minist Educ, Engn Res Ctr Mol & Neuro Imaging, Xian 710071, Shaanxi, Peoples R China 2.Fourth Mil Med Univ, Tangdu Hosp, Dept Gynecol & Obstet, Xian 710038, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fu,Zhang, Beilei,Zhou, Lin,et al. Imaging Dendrimer-Grafted Graphene Oxide Mediated Anti-miR-21 Delivery With an Activatable Luciferase Reporter[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(14):9014-9021. |
| APA | Wang, Fu.,Zhang, Beilei.,Zhou, Lin.,Shi, Yaru.,Li, Zhiqiang.,...&Tian, Jie.(2016).Imaging Dendrimer-Grafted Graphene Oxide Mediated Anti-miR-21 Delivery With an Activatable Luciferase Reporter.ACS APPLIED MATERIALS & INTERFACES,8(14),9014-9021. |
| MLA | Wang, Fu,et al."Imaging Dendrimer-Grafted Graphene Oxide Mediated Anti-miR-21 Delivery With an Activatable Luciferase Reporter".ACS APPLIED MATERIALS & INTERFACES 8.14(2016):9014-9021. |
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来源：自动化研究所
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