Volumetric chemical imaging by stimulated Raman projection microscopy and tomography
文献类型：期刊论文
| 作者 | Chen, Xueli1,2,3; Zhang, Chi3; Lin, Peng3 ; Huang, Kai-Chih3; Liang, Jimin1,2; Tian, Jie1,2,4; Cheng, Ji-Xin3,5
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| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2017-04-24 |
| 卷号 | 8 |
| DOI | 10.1038/ncomms15117 |
| 文献子类 | Article |
| 英文摘要 | Volumetric imaging allows global understanding of three-dimensional (3D) complex systems. Light-sheet fluorescence microscopy and optical projection tomography have been reported to image 3D volumes with high resolutions and at high speeds. Such methods, however, usually rely on fluorescent labels for chemical targeting, which could perturb the biological functionality in living systems. We demonstrate Bessel-beam-based stimulated Raman projection (SRP) microscopy and tomography for label-free volumetric chemical imaging. Our SRP microscope enables fast quantitation of chemicals in a 3D volume through a two-dimensional lateral scan. Furthermore, combining SRP and sample rotation, we demonstrate the SRP tomography that can reconstruct the 3D distribution of chemical compositions with optical spatial resolution at a higher speed than the Gaussian-beam-based stimulated Raman scattering sectioning imaging can. We explore the potential of our SRP technology by mapping polymer particles in 3D volumes and lipid droplets in adipose cells. |
| WOS关键词 | LIGHT-SHEET MICROSCOPY ; PLANE ILLUMINATION MICROSCOPY ; SCATTERING MICROSCOPY ; IN-VIVO ; RESOLUTION ; SPECIMENS ; BIOLOGY ; BEAMS |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000400112500001 |
| 资助机构 | Keck Foundation ; National Institutes of Health (NIH)(GM118471) ; National Natural Science Foundation of China(81627807 ; China Scholarship Council(201406965025) ; Tianjin University ; 81227901 ; 81571725) |
| 源URL | [http://ir.ia.ac.cn/handle/173211/15096]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 作者单位 | 1.Xidian Univ, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710126, Shaanxi, Peoples R China 2.Xidian Univ, Sch Life Sci & Technol, Xian 710126, Shaanxi, Peoples R China 3.Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA 4.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China 5.Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA |
| 推荐引用方式 GB/T 7714 | Chen, Xueli,Zhang, Chi,Lin, Peng,et al. Volumetric chemical imaging by stimulated Raman projection microscopy and tomography[J]. NATURE COMMUNICATIONS,2017,8. |
| APA | Chen, Xueli.,Zhang, Chi.,Lin, Peng.,Huang, Kai-Chih.,Liang, Jimin.,...&Cheng, Ji-Xin.(2017).Volumetric chemical imaging by stimulated Raman projection microscopy and tomography.NATURE COMMUNICATIONS,8. |
| MLA | Chen, Xueli,et al."Volumetric chemical imaging by stimulated Raman projection microscopy and tomography".NATURE COMMUNICATIONS 8(2017). |
入库方式： OAI收割
来源：自动化研究所
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