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The interactions of single-wall carbon nanohorns with polar epithelium

文献类型：期刊论文

作者Shi, Yujie1; Shi, Zujin2; Li, Suxin1; Zhang, Yuan3; He, Bing1; Peng, Dong4; Tian, Jie4; Zhao, Ming5; Wang, Xueqing1; Zhang, Qiang1
刊名INTERNATIONAL JOURNAL OF NANOMEDICINE
出版日期2017
卷号12页码:4177-4194
关键词Carbon Nanohorns Epithelial Cells Transport Mechanisms Nanocarriers Msot Imaging
DOI10.2147/IJN.S133295
文献子类Article
英文摘要Single-wall carbon nanohorns (SWCNHs), which have multitudes of horn interstices, an extensive surface area, and a spherical aggregate structure, offer many advantages over other carbon nanomaterials being used as a drug nanovector. The previous studies on the interaction between SWCNHs and cells have mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells. Polar epithelium as a typical biological barrier constitutes the prime obstacle for the transport of therapeutic agents to target site. This work tried to explore the permeability of SWCNHs through polar epithelium and their abilities to modulate transcellular transport, and evaluate the potential of SWCNHs in drug delivery. Madin-Darby canine kidney (MDCK) cell monolayer was used as a polar epithelial cell model, and as-grown SWCNHs, together with oxidized and fluorescein isothiocyanate-conjugated bovine serum albumin-labeled forms, were constructed and comprehensively investigated in vitro and in vivo. Various methods such as transmission electron microscopy and confocal imaging were used to visualize their intracellular uptake and localization, as well as to investigate the potential transcytotic process. The related mechanism was explored by specific inhibitors. Additionally, fast multispectral optoacoustic tomography imaging was used for monitoring the distribution and transport process of SWCNHs in vivo after oral administration in nude mice, as an evidence for their interaction with the intestinal epithelium. The results showed that SWCNHs had a strong bioadhesion property, and parts of them could be uptaken and transcytosed across the MDCK monolayer. Multiple mechanisms were involved in the uptake and transcytosis of SWCNHs with varying degrees. After oral administration, oxidized SWCNHs were distributed in the gastrointestinal tract and retained in the intestine for up to 36 h probably due to their surface adhesion and endocytosis into the intestinal epithelium. Overall, this comprehensive investigation demonstrated that SWCNHs can serve as a promising nanovector that can cross the barrier of polar epithelial cells and deliver drugs effectively.
WOS关键词IN-VIVO ; POLYMERIC MICELLES ; TIGHT JUNCTIONS ; DRUG-DELIVERY ; MDCK CELLS ; NANOPARTICLES ; TRANSPORT ; ACCUMULATION ; NANOCARRIERS ; PROTEINS
WOS研究方向Science & Technology - Other Topics ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000402665500004
资助机构National Basic Research Program of China (973 program)(2015CB932100) ; National Natural Science Foundation of China(81603041)
源URL[http://ir.ia.ac.cn/handle/173211/15131]  
专题自动化研究所_中国科学院分子影像重点实验室
作者单位1.Peking Univ, Sch Pharmaceut Sci, Coll Chem & Mol Engn, Beijing Key Lab Mol Pharmaceut & New Drug Deliver, Beijing, Peoples R China
2.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing, Peoples R China
3.Univ Rhode Isl, Coll Pharm, Dept Biomed & Pharmaceut Sci, Kingston, RI 02881 USA
4.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing, Peoples R China
5.Peking Univ, Sch Pharmaceut Sci, Dept Chem Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714		 Shi, Yujie,Shi, Zujin,Li, Suxin,et al. The interactions of single-wall carbon nanohorns with polar epithelium[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2017,12:4177-4194.
APA Shi, Yujie.,Shi, Zujin.,Li, Suxin.,Zhang, Yuan.,He, Bing.,...&Zhang, Qiang.(2017).The interactions of single-wall carbon nanohorns with polar epithelium.INTERNATIONAL JOURNAL OF NANOMEDICINE,12,4177-4194.
MLA Shi, Yujie,et al."The interactions of single-wall carbon nanohorns with polar epithelium".INTERNATIONAL JOURNAL OF NANOMEDICINE 12(2017):4177-4194.

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来源：自动化研究所

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