Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation
文献类型：期刊论文
| 作者 | Du Y (杜洋) ; Yip H
|
| 刊名 | Differentiation
 |
| 出版日期 | 2010-02 |
| 卷号 | 79期号:2页码:84 |
| 关键词 | Bone Morphogenetic Protein 2 (Bmp2) Id1-3 Differentiation |
| 英文摘要 | Bone morphogenetic proteins (BMPs) are secretory signal molecules that have a variety of regulatory functions during embryonic morphogenesis. BMP2 has been shown to induce differentiation in many cell types, mediated through the activation of its target genes: the inhibitors of differentiation (Id1-3) and key transcription factors. In this study, we investigated the effects of BMP2 on mouse neuroblastoma (Neuro2a) cell differentiation and regulation of the expression of Id1-3 and neural-specific transcription factors. Our results showed that BMP2 stimulation upregulated Id1-3 expression at the early stage of application by involvement of the Smad signaling pathway. BMP2 caused phosphorylation of Smad1/5/8 followed by upregulation of Id1-3. Co-incubation with Noggin, a BMP antagonist, or Smad1 siRNA transfection significantly inhibited phosphorylation of Smad1/5/8 and upregulation of Id protein. Furthermore, our results showed that BMP2-induced differentiation of Neuro2a cells into neurons by downregulating the expression of Id1-3 proteins and upregulating the expression of neural-specific transcriptional factors Dlx2, Brn3a, and NeuroD6. The results suggested that the transient upregulation of Id1-3 expression during the early phase of BMP stimulation may play a role in lineage specification and promote differentiation of neuroblastoma cells towards a neuronal phenotype. Subsequently, a coordinated increase in expression of proneural transcription factors and a decrease in Id1-3 expression may culminate in the transition from proliferation to neurogenesis and the terminal neuronal differentiation of neuroblastoma cells. |
| 源URL | [http://ir.ia.ac.cn/handle/173211/20496]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 推荐引用方式 GB/T 7714 | Du Y ,Yip H. Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation[J]. Differentiation,2010,79(2):84. |
| APA | Du Y ,&Yip H.(2010).Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation.Differentiation,79(2),84. |
| MLA | Du Y ,et al."Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation".Differentiation 79.2(2010):84. |
入库方式： OAI收割
来源：自动化研究所
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