Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture
文献类型：期刊论文
| 作者 | Jia Xiaohua
|
| 刊名 | Chin J Integr Med
 |
| 出版日期 | 2013-08 |
| 卷号 | 19期号:8页码:573 |
| 关键词 | Electroacupuncture |
| 英文摘要 | ; Objective: To investigate whether electroacupuncture (EA) can promote cell survival and enhance heart function of mesenchymal stem cells (MSCs) therapy. Methods: MSCs were isolated from bone marrow and expanded in Minimum Essential Medium Alpha (α-MEM). MI was induced in 72 Sprague-Dawley (S-D) rats by ligation of the left anterior descending coronary artery (LAD) for 30 min and reperfusion. MI rats randomly received injection of 1×106 DiI-labeled MSCs alone (n=24, MSC group), or plus electroacupuncture (EA) at Neiguan (PC6, n=24, EA+MSC group), or saline (n=24, saline group). EA treatment was performed for 4 days. Another 24 rats were subjected to chest-open surgery without LAD occlusion and treatment (sham group). Three time points, 4, 14 and 28 days (n=8 for each group) were included in this study. The survival of transplanted MSCs and the protective gene expression were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot at day 4 and 14. Left ventricular remodeling, cardiac function, infarction area, fibrosis and capillary density were analyzed at day 28. Results: EA can enhance MSC survival (2.6-fold up) at day 4. Big capillary density was 53% higher in EA+MSC treated group than MSC alone group. Furthermore, the rats treated by EA reduced the fibrosis and had 36% smaller infarct size comparing to MSC alone. EA also attenuated left ventricular remodeling and enhanced the functional recovery of infarcted hearts at week 4. Conclusion: EA at Neiguan acupoint can promote the stem cell survival and improve ischemic heart function. EA could become a useful approach in stem cell therapy for ischemia heart diseases. |
| 源URL | [http://ir.ia.ac.cn/handle/173211/21794]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 推荐引用方式 GB/T 7714 | Jia Xiaohua. Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture[J]. Chin J Integr Med,2013,19(8):573. |
| APA | Jia Xiaohua.(2013).Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture.Chin J Integr Med,19(8),573. |
| MLA | Jia Xiaohua."Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture".Chin J Integr Med 19.8(2013):573. |
入库方式： OAI收割
来源：自动化研究所
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