Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter
文献类型：期刊论文
| 作者 | Shi, Yaru1; Liu, Wei2 ; Zheng, Haifeng1; Li, Zhiqiang1; Shi, Xiaorui1; Jiao, Zhiqiang1; Mao, Wenjie1; Xie, Jinrong1; Tian, Jie1,3; Wang, Fu1
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| 刊名 | BIOMEDICAL OPTICS EXPRESS
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 9期号:2页码:518-528 |
| DOI | 10.1364/BOE.9.000518 |
| 文献子类 | Article |
| 英文摘要 | Pre-mRNA splicing is an essential step in gene expression in most eukaryote genes. Here we present the feasibility of a genetically encoded luciferase reporter to monitor the pre-mRNA splicing process in living cells and animals. We showed that the splicing activity change induced by isoginkgetin could be readily visualized in vitro both in a dose and time dependent manner. Moreover, the pre-mRNA splicing process could be also obviously detected in mice by bioluminescence imaging and confirmed by RT-PCR. Our work provided a reporter system that allows high-throughput screening of chemical libraries to identify potential compounds leading to aberrant patterns of splicing. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| WOS关键词 | MAMMALIAN-CELLS ; ANTISENSE OLIGONUCLEOTIDES ; IN-VIVO ; SPLICEOSOME ; EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000425923300013 |
| 资助机构 | National Natural Science Foundation of China(81571721 ; Natural Science Basis Research Plan in Shaanxi Province of China(2016JM8016) ; 81772010) |
| 源URL | [http://ir.ia.ac.cn/handle/173211/21955]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 作者单位 | 1.Xidian Univ, Sch Life Sci & Technol, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710071, Shaanxi, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp, Dept Hepatobiliary Surg, Xian 710032, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yaru,Liu, Wei,Zheng, Haifeng,et al. Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter[J]. BIOMEDICAL OPTICS EXPRESS,2018,9(2):518-528. |
| APA | Shi, Yaru.,Liu, Wei.,Zheng, Haifeng.,Li, Zhiqiang.,Shi, Xiaorui.,...&Wang, Fu.(2018).Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter.BIOMEDICAL OPTICS EXPRESS,9(2),518-528. |
| MLA | Shi, Yaru,et al."Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter".BIOMEDICAL OPTICS EXPRESS 9.2(2018):518-528. |
入库方式： OAI收割
来源：自动化研究所
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