Phage Display-Derived Peptide-based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Post-Instillation: A Preclinical Study
文献类型：期刊论文
| 作者 | Li Peng1,2,4; Wenting Shang2,3 ; Pengyu Guo1,2,4; Kunshan He2,3; Hongzhi Wang1,4; Ziyu Han2,3; Hongmei Jiang2,3; Jie Tian2,3,5; Kun Wang2,3; Wanhai Xu1,4
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| 刊名 | Molecular Cancer Therapeutics
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| 出版日期 | 2018-10 |
| 期号 | 17页码:2100-2111 |
| 关键词 | Bladder Cancer Diagnosis And Resection Preclinical Study |
| 英文摘要 | Bladder cancer (BC) is a common human malignancy. Conventional ultrasound and white light cystoscopy are often used for BC diagnosis and resection, but insufficient specificity results in a high BC recurrence rate. New strategy for the diagnosis and resection of BC are needed. In this study, we developed a highly specific peptide-based probe for BC photoacoustic imaging (PAI) diagnosis and near-infrared (NIR)-imaging-guided resection post-instillation. A BC-specific peptide (PLSWT7) was selected by in vivo phage display technology and labeled with IRDye800CW to synthesize a BC-specific dual-modality imaging (DMI) probe (PLSWT7-DMI). The feasibility of PLSWT7-DMI-based dual-modality PAI-NIR imaging was assessed in vitro, in mouse models, and ex vivo human bladders. An air-pouch BC (APBC) model suitable for probe instillation was established to evaluate the probe-based BC PAI diagnosis and NIR-imaging-guided resection. Human bladders were used to assess whether PLSWT7-DMI-based DMI strategy is a translatable approach for BC detection and resection. The probe exhibited excellent selectivity and specificity both in vitro and in vivo. Post-instillation of the probe, tumors <3 mm were detectable by PAI, and NIR-imaging-guided tumor resection decreased the BC recurrence rate by 90% and increased the survival in the mouse model. Additionally, ex vivo NIR imaging of human bladders indicated that PLSWT7-DMI-based imaging would potentially allowed precise resection of BC in clinical settings. This PLSWT7-DMI-based DMI strategy was a translatable approach for BC diagnosis and resection and could potentially lower the BC recurrence rate. |
| 源URL | [http://ir.ia.ac.cn/handle/173211/22026]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 作者单位 | 1.Urology Surgery Department, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P. R. China. 2.CAS Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, P. R. China. 3.The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China. 4.Heilongjiang Key Laboratory of Scientific Research in Urology, Harbin, Heilongjiang 150001, P. R. China. 5.Beihang University, Beijing 100190, P. R. China |
| 推荐引用方式 GB/T 7714 | Li Peng,Wenting Shang,Pengyu Guo,et al. Phage Display-Derived Peptide-based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Post-Instillation: A Preclinical Study[J]. Molecular Cancer Therapeutics,2018(17):2100-2111. |
| APA | Li Peng.,Wenting Shang.,Pengyu Guo.,Kunshan He.,Hongzhi Wang.,...&Wanhai Xu.(2018).Phage Display-Derived Peptide-based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Post-Instillation: A Preclinical Study.Molecular Cancer Therapeutics(17),2100-2111. |
| MLA | Li Peng,et al."Phage Display-Derived Peptide-based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Post-Instillation: A Preclinical Study".Molecular Cancer Therapeutics .17(2018):2100-2111. |
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来源：自动化研究所
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