Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM
文献类型：期刊论文
| 作者 | Li, Xixia1,3; Ji, Gang1,3,4; Chen, Xi2 ; Ding, Wei1,3; Sun, Lei1,3; Xu, Wei1; Han, Hua2; Sun, Fei1,3,4
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| 刊名 | JOURNAL OF STRUCTURAL BIOLOGY
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| 出版日期 | 2017-11-01 |
| 卷号 | 200期号:2页码:87-96 |
| 关键词 | Automation Caenorhabditis Elegans Scanning Electron Microscopy 3d Reconstruction Volume Electron Microscopy |
| DOI | 10.1016/j.jsb.2017.09.010 |
| 文献子类 | Article |
| 英文摘要 | Large scale, high resolution three dimensional (3D) ultrastructural reconstruction of cells and tissues has become increasingly important to our understanding of complex biological systems. There have been a few partial 3D ultra-structures of Caenorhabditis elegans (C. elegans ) reported, however 3D reconstruction of a whole nematode has never been achieved. Here, we independently developed a technique called automatic collector of ultrathin sections scanning electron microscopy and using this methodology, generated a 3D reconstruction of an entire C. elegans larva with 100 nm axial and 15 nm lateral resolution. Compared to the current available ATUM (automated tape-collecting ultramicrotome) technique, our work provides another alternative complete solution that can be applied to obtain large scale 3D ultrastructure of tissues. Our workflow includes an automated hardware system for high throughput serial section collection, a software package for automatic SEM imaging, and an image reconstruction program. These combined techniques can now be used together to rapidly provide access to understand the anatomy of the whole nematodes. |
| WOS关键词 | NEMATODE CAENORHABDITIS-ELEGANS ; VOLUME ELECTRON-MICROSCOPY ; C-ELEGANS ; HIGH-RESOLUTION ; NERVOUS-SYSTEM ; FINE-STRUCTURE ; NEURONS ; PRESERVATION ; NEOCORTEX ; BIOLOGY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000416299300002 |
| 资助机构 | foundation of IBP & Leica Microsystems Ltd Joint Laboratory ; Equipment Development&Technology Innovation Programs of the Chinese Academy of Sciences(Y6CZ021001) ; National Natural Science Foundation of China(31501160 ; National Key Foundation for Exploring Scientific Instruments of China(2013YQ12035311) ; 31470838) |
| 源URL | [http://ir.ia.ac.cn/handle/173211/21919]  |
| 专题 | 类脑智能研究中心_微观重建与智能分析 |
| 作者单位 | 1.Chinese Acad Sci, Ctr Biol Imaging, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst Biophydcs, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China 4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xixia,Ji, Gang,Chen, Xi,et al. Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM[J]. JOURNAL OF STRUCTURAL BIOLOGY,2017,200(2):87-96. |
| APA | Li, Xixia.,Ji, Gang.,Chen, Xi.,Ding, Wei.,Sun, Lei.,...&Sun, Fei.(2017).Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM.JOURNAL OF STRUCTURAL BIOLOGY,200(2),87-96. |
| MLA | Li, Xixia,et al."Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM".JOURNAL OF STRUCTURAL BIOLOGY 200.2(2017):87-96. |
入库方式： OAI收割
来源：自动化研究所
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