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LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent
文献类型：期刊论文
| 作者 | Ding, Chenxu1,4; Wang, Lingyun1,5; Tian, Chunliang2; Li, Yulin1; Sun, Zhiwei1,5; Wang, Honglun1; Suo, Yourui1; You, Jinmao1,3 |
| 刊名 | chromatographia
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 68期号:11-12页码:893-902 |
| 关键词 | Column liquid chromatography-mass spectrometry Carbohydrates 1-(2-Naphthyl)-3-methyl-5-pyrazolone |
| 合作状况 | 其它 |
| 中文摘要 | a novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (nmp) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. carbohydrates are derivatized to their bis-nmp-labeled derivatives. derivatives showed an intense protonated molecular ion at m/z [m+h](+) in positive-ion detection mode. the mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. this characteristic fragment ion is from the cleavage of c2-c3 bond in carbohydrate chain giving the specific fragment ions at m/z [mh-cmh2m+1om-h2o](+) for pentose, hexose and glyceraldehydes and at m/z [mh-cmh2m-1om+1-h2o](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). no interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. the method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase hypersil ods-2 column. the carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected. |
| 英文摘要 | a novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (nmp) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. carbohydrates are derivatized to their bis-nmp-labeled derivatives. derivatives showed an intense protonated molecular ion at m/z [m+h](+) in positive-ion detection mode. the mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. this characteristic fragment ion is from the cleavage of c2-c3 bond in carbohydrate chain giving the specific fragment ions at m/z [mh-c(m)h(2m+1)o(m)-h(2)o](+) for pentose, hexose and glyceraldehydes and at m/z [mh-c(m)h(2m-1)o(m+1)-h(2)o](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). no interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. the method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase hypersil ods-2 column. the carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected. |
| 学科主题 | 生物科学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; tandem mass-spectrometry ; fast-atom-bombardment ; capillary-electrophoresis ; oligosaccharides ; derivatives ; sugars ; acid ; glycoproteins ; electrospray |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261422300003 |
| 公开日期 | 2009-12-04 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1187]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Jining Univ, Dept Chem, Jining 273155, Shandong, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 4.Qinghai Nationalities Inst, Dept Pharm, Xining 810007, Qinghai, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Chenxu,Wang, Lingyun,Tian, Chunliang,et al. LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent[J]. chromatographia,2008,68(11-12):893-902. |
| APA | Ding, Chenxu.,Wang, Lingyun.,Tian, Chunliang.,Li, Yulin.,Sun, Zhiwei.,...&You, Jinmao.(2008).LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent.chromatographia,68(11-12),893-902. |
| MLA | Ding, Chenxu,et al."LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent".chromatographia 68.11-12(2008):893-902. |
入库方式： OAI收割
来源：西北高原生物研究所
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