Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells
文献类型：期刊论文
| 作者 | Shi, Ping1,2; Huang, Zhiwei1,3; Chen, Guichen1 |
| 刊名 | american journal of chinese medicine
 |
| 出版日期 | 2008 |
| 卷号 | 36期号:4页码:805-813 |
| 关键词 | rhein human hepatocelluar carcinoma BEL-7402 cells apoptosis cell cycle arrest c-Myc caspase-3 |
| 合作状况 | 其它 |
| 中文摘要 | rhein, an anthraquinone derivative of rhubarb, inhibits the proliferation of various human cancer cells. in this paper, we focused on studying the effects of rhein on human hepatocelluar carcinoma bel-7402 cells and further understanding the underlying molecular mechanism in an effort to make the potential development of rhein in the treatment of cancers. using mtt assay and flow cytometry, we demonstrate a critical role of rhein in the suppression of bel-7402 cell proliferation in a concentration- and time-dependent manner. the increase of apoptosis rate was observed after incubation of bel-7402 cells with rhein at 50-200 mu m for 48 hours, and the cells exhibit typical apoptotic features including cellular morphological change and chromatin condensation. moreover, rhein-induced cell cycle s-phase arrest. additionally, after rhein treatment, expression levels of c-myc gene were decreased, while those of caspase-3 gene were increased in a dose-dependent manner by using real-time pcr assay. the results demonstrate for the first time that cell cycle s-phase arrest is one of the mechanisms of rhein in inhibition of bel-7402 cells. rhein plays its role by inducing cell cycle arrest via downregulation of oncogene c-myc and apoptosis through the caspase-dependent pathway. it is expected that rhein will be effective and useful as a new agent in hepatocelluar carcinoma treatment in the future. |
| 英文摘要 | rhein, an anthraquinone derivative of rhubarb, inhibits the proliferation of various human cancer cells. in this paper, we focused on studying the effects of rhein on human hepatocelluar carcinoma bel-7402 cells and further understanding the underlying molecular mechanism in an effort to make the potential development of rhein in the treatment of cancers. using mtt assay and flow cytometry, we demonstrate a critical role of rhein in the suppression of bel-7402 cell proliferation in a concentration- and time-dependent manner. the increase of apoptosis rate was observed after incubation of bel-7402 cells with rhein at 50-200 mu m for 48 hours, and the cells exhibit typical apoptotic features including cellular morphological change and chromatin condensation. moreover, rhein-induced cell cycle s-phase arrest. additionally, after rhein treatment, expression levels of c-myc gene were decreased, while those of caspase-3 gene were increased in a dose-dependent manner by using real-time pcr assay. the results demonstrate for the first time that cell cycle s-phase arrest is one of the mechanisms of rhein in inhibition of bel-7402 cells. rhein plays its role by inducing cell cycle arrest via downregulation of oncogene c-myc and apoptosis through the caspase-dependent pathway. it is expected that rhein will be effective and useful as a new agent in hepatocelluar carcinoma treatment in the future. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 药学 |
| 类目[WOS] | integrative & complementary medicine ; medicine, general & internal |
| 研究领域[WOS] | integrative & complementary medicine ; general & internal medicine |
| 关键词[WOS] | natural-products ; cancer |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258793200016 |
| 公开日期 | 2009-12-04 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1197]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China 2.Univ Texas MD Anderson Canc Ctr, Dept Hematopathol, Houston, TX 77030 USA 3.Baylor Coll Med, Houston, TX 77030 USA |
| 推荐引用方式 GB/T 7714 | Shi, Ping,Huang, Zhiwei,Chen, Guichen. Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells[J]. american journal of chinese medicine,2008,36(4):805-813. |
| APA | Shi, Ping,Huang, Zhiwei,&Chen, Guichen.(2008).Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells.american journal of chinese medicine,36(4),805-813. |
| MLA | Shi, Ping,et al."Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells".american journal of chinese medicine 36.4(2008):805-813. |
入库方式： OAI收割
来源：西北高原生物研究所
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