Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification
文献类型：期刊论文
| 作者 | Zhao, Xianen1,2; Suo, Yourui1,2; Wang, Lingyun1,2; You, Jinmao1,3; Ding, Chenxu1,3 |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2008 |
| 卷号 | 31期号:16页码:2375-2400 |
| 关键词 | 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) carbohydrates derivatization Herpetospermum pedunculosum (Ser.) Baill liquid chromatography-mass spectrometry (ESI/MS) |
| 合作状况 | 其它 |
| 中文摘要 | a new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone (nmp), coupling with liquid chromatography (lc) with electrospray ionization mass spectrometry (esi-ms) for the detection of carbohydrates from a famous tibetan medicine is reported. carbohydrates were derivatized to their bis-nmp-labeled derivatives. the method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed phase hypersil ods-2 column. the carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose, and fucose could be successfully detected by uv and esi-ms. derivatives showed intense protonated molecular ion at m/z [m+h]+ in positive ion mode. the mass to charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative identification of carbohydrates; this characteristic fragment ion was from the cleavage of c2-c3 bond in the carbohydrate chain giving the specific fragment ions at m/z [mh-cmh2m+1om-h2o](+) for pentose, hexose, and glyceraldehydes, and at m/z [mh-cmh2m-1om+1-h2o](+) for alduronic acids, such as galacturonic acid and glucuronic acid (m=n-2, n is carbon atom number of carbohydrate). compared with the traditional 1-phenyl-3-methyl-5-pyrazolone (pmp) reagent, currently synthesized nmp show the advantage of higher sensitivity to carbohydrate compounds with uv and esi-ms detection. |
| 英文摘要 | a new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone (nmp), coupling with liquid chromatography (lc) with electrospray ionization mass spectrometry (esi-ms) for the detection of carbohydrates from a famous tibetan medicine is reported. carbohydrates were derivatized to their bis-nmp-labeled derivatives. the method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed phase hypersil ods-2 column. the carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose, and fucose could be successfully detected by uv and esi-ms. derivatives showed intense protonated molecular ion at m/z [m+h]+ in positive ion mode. the mass to charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative identification of carbohydrates; this characteristic fragment ion was from the cleavage of c2-c3 bond in the carbohydrate chain giving the specific fragment ions at m/z [mh-c(m)h(2m+1)o(m)-h(2)o](+) for pentose, hexose, and glyceraldehydes, and at m/z [mh-c(m)h(2m-1)o(m+1)-h(2)o](+) for alduronic acids, such as galacturonic acid and glucuronic acid (m=n-2, n is carbon atom number of carbohydrate). compared with the traditional 1-phenyl-3-methyl-5-pyrazolone (pmp) reagent, currently synthesized nmp show the advantage of higher sensitivity to carbohydrate compounds with uv and esi-ms detection. |
| 学科主题 | 生物科学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; herpetospermum-caudigerum wall ; desorption/ionization mass-spectrometry ; oligosaccharides ; derivatives ; derivatization ; electrospray ; separation ; acid ; monosaccharide |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258622400002 |
| 公开日期 | 2009-12-04 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1198]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xianen,Suo, Yourui,Wang, Lingyun,et al. Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification[J]. journal of liquid chromatography & related technologies,2008,31(16):2375-2400. |
| APA | Zhao, Xianen,Suo, Yourui,Wang, Lingyun,You, Jinmao,&Ding, Chenxu.(2008).Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification.journal of liquid chromatography & related technologies,31(16),2375-2400. |
| MLA | Zhao, Xianen,et al."Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification".journal of liquid chromatography & related technologies 31.16(2008):2375-2400. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。