中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)

文献类型:期刊论文

作者Wang, Ailan1; Zhang, Qian1; Wan, Dongshi1; Yang, Yanzhuo1; Liu, Jianquan1,2
刊名conservation genetics
出版日期2008
卷号9期号:4页码:969-971
关键词Hippophae rhamnoides ssp sinensis microsatellite markers population genetics
合作状况其它
中文摘要hippophae rhamnoides ssp. sinensis occurs mainly in the and regions of northwest china. the wood stands of this subspecies play an important role in maintaining the local ecosystems in these regions. in addition, the genetic characteristics are essential to understand the historical range changes of this subspecies and its morphological differentiation with other subspecies. in this study, we developed nine microsatellite loci for this subspecies for the first time. we used the combining biotin capture method to enrich ag/ct/ac/gt/cg/gtg/cca microsatellites. twenty-six microsatellites were isolated from the enriching library and nine of them were found to be polymorphic through screening 12 distantly distributed individuals. the number of alleles per locus ranged from three to twelve and expected heterozygosity from 0.2659 to 0.4767, respectively. we further performed cross-priming tests in another subspecies and two congeneric species. these firstly isolated loci will provide a useful tool to investigate the genetic structure of this subspecies and its morphological differentiation from the other subspecies.
英文摘要hippophae rhamnoides ssp. sinensis occurs mainly in the and regions of northwest china. the wood stands of this subspecies play an important role in maintaining the local ecosystems in these regions. in addition, the genetic characteristics are essential to understand the historical range changes of this subspecies and its morphological differentiation with other subspecies. in this study, we developed nine microsatellite loci for this subspecies for the first time. we used the combining biotin capture method to enrich ag/ct/ac/gt/cg/gtg/cca microsatellites. twenty-six microsatellites were isolated from the enriching library and nine of them were found to be polymorphic through screening 12 distantly distributed individuals. the number of alleles per locus ranged from three to twelve and expected heterozygosity from 0.2659 to 0.4767, respectively. we further performed cross-priming tests in another subspecies and two congeneric species. these firstly isolated loci will provide a useful tool to investigate the genetic structure of this subspecies and its morphological differentiation from the other subspecies.
WOS标题词science & technology ; life sciences & biomedicine
学科主题生物科学
类目[WOS]biodiversity conservation ; genetics & heredity
研究领域[WOS]biodiversity & conservation ; genetics & heredity
收录类别SCI
语种英语
WOS记录号WOS:000257420700022
公开日期2009-12-04
源URL[http://ir.nwipb.ac.cn/handle/363003/1207]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Lanzhou Univ, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Qinghai Tibetan Plateau Biol Evolut & Ada, Xining, Qinghai, Peoples R China
推荐引用方式
GB/T 7714
Wang, Ailan,Zhang, Qian,Wan, Dongshi,et al. Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)[J]. conservation genetics,2008,9(4):969-971.
APA Wang, Ailan,Zhang, Qian,Wan, Dongshi,Yang, Yanzhuo,&Liu, Jianquan.(2008).Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae).conservation genetics,9(4),969-971.
MLA Wang, Ailan,et al."Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)".conservation genetics 9.4(2008):969-971.

入库方式: OAI收割

来源:西北高原生物研究所

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