LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology
文献类型：期刊论文
| 作者 | Zhao, Xianen1,2; Suo, Yourui1 |
| 刊名 | chromatographia
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 67期号:5-6页码:375-382 |
| 关键词 | column liquid chromatography-mass spectrometry amino acids in rat plasma 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate |
| 合作状况 | 其它 |
| 中文摘要 | an lc method for the determination of 20 amino acids (aas), using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (bceoc) as fluorescent labeling reagent, has been validated and applied for the analysis of aas in rat plasma at three different states concerning exercise physiology. identification of aa derivatives was carried out by lc-ms with electrospray ion (esi), and the ms-ms cleavage mode of the representative tyrosine (tyr) derivative was analyzed. gradient elution on a hypersil bds c-18 column gave good separation of the derivatives. excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu l of plasma samples. the contents of 20 aas in rat plasma of three groups (24 rats, group a: quiet state, group b: at exercising exhaust, group c: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. facile bceoc derivatization coupled with lc-fld-esi-ms analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of aas from plasma or other biochemical samples. |
| 英文摘要 | an lc method for the determination of 20 amino acids (aas), using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (bceoc) as fluorescent labeling reagent, has been validated and applied for the analysis of aas in rat plasma at three different states concerning exercise physiology. identification of aa derivatives was carried out by lc-ms with electrospray ion (esi), and the ms-ms cleavage mode of the representative tyrosine (tyr) derivative was analyzed. gradient elution on a hypersil bds c-18 column gave good separation of the derivatives. excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu l of plasma samples. the contents of 20 aas in rat plasma of three groups (24 rats, group a: quiet state, group b: at exercising exhaust, group c: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. facile bceoc derivatization coupled with lc-fld-esi-ms analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of aas from plasma or other biochemical samples. |
| 学科主题 | 生物科学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; mass-spectrometry ; 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate ; precolumn derivatization ; hepatitis ; fatigue ; reagent |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253624100005 |
| 公开日期 | 2009-12-07 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1231]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xianen,Suo, Yourui. LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology[J]. chromatographia,2008,67(5-6):375-382. |
| APA | Zhao, Xianen,&Suo, Yourui.(2008).LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology.chromatographia,67(5-6),375-382. |
| MLA | Zhao, Xianen,et al."LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology".chromatographia 67.5-6(2008):375-382. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。