CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides
文献类型：期刊论文
| 作者 | Ding, Chenxu1,3; Sun, Xuejun2; Zhao, Xianen1,4; Zhao, Wenchen1,2; Li, Yulin1; Wang, Honglun1; Suo, Yourui1; You, Jinmao1,2 |
| 刊名 | chromatographia
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 67期号:3-4页码:245-251 |
| 关键词 | capillary electrophoresis 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) oligopeptides derivatization |
| 合作状况 | 其它 |
| 中文摘要 | a pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (ceoc-cl) followed by capillary electrophoresis (ce) with diode-array detection, has been developed. maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at ph 9.0-10.0. excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using ce with no significant disturbance from the major fluorescent reagent degradation by-products. the effects on the results of buffer ph and of sds and organic modifier concentrations were examined. good baseline resolution in the separation of five ceoc-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-mu m inner diameter capillary column. |
| 英文摘要 | a pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (ceoc-cl) followed by capillary electrophoresis (ce) with diode-array detection, has been developed. maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at ph 9.0-10.0. excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using ce with no significant disturbance from the major fluorescent reagent degradation by-products. the effects on the results of buffer ph and of sds and organic modifier concentrations were examined. good baseline resolution in the separation of five ceoc-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-mu m inner diameter capillary column. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 生物科学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; capillary-zone-electrophoresis ; amino-acid-analysis ; micellar electrokinetic chromatography ; induced fluorescence detection ; 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate ; naphthalene-2,3-dicarboxaldehyde derivatization ; 2-(9-anthryl)ethyl chloroformate ; precolumn derivatization ; mass-spectrometry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000252806200007 |
| 公开日期 | 2009-12-07 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1238]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China 3.Qinghai Nationalities Inst, Dept Pharm, Xining 810007, Qinghai, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Chenxu,Sun, Xuejun,Zhao, Xianen,et al. CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides[J]. chromatographia,2008,67(3-4):245-251. |
| APA | Ding, Chenxu.,Sun, Xuejun.,Zhao, Xianen.,Zhao, Wenchen.,Li, Yulin.,...&You, Jinmao.(2008).CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides.chromatographia,67(3-4),245-251. |
| MLA | Ding, Chenxu,et al."CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides".chromatographia 67.3-4(2008):245-251. |
入库方式： OAI收割
来源：西北高原生物研究所
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