Analysis of five pharmacologically active compounds from the Tibetan medicine Elsholtzia with micellar electrokinetic capillary chromatography
文献类型：期刊论文
| 作者 | Ding, Chenxu; Wang, Lingyun; Zhao, Xianen; Li, Yulin; Wang, Honglun; You, Jinmao; Suo, Yourui |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2007 |
| 卷号 | 30期号:20页码:3069-3083 |
| 关键词 | micellar electrokinetic capillary chromatography Elsholtzia oleanolic acid kaempferol saussurenoside quercetin rutin |
| 合作状况 | 其它 |
| 中文摘要 | a high performance capillary electrophoresis method with diode array detector detection for the determination of five bioactive ingredients in tibetan medicine elsholtzia, namely quercetin, rutin, saussurenoside, kaempferol, and oleanolic acid, has been developed. the effects of several factors, such as the acidity, concentration of running buffer, separation voltage, temperature, and sds concentration were investigated. the optimal conditions were 44 mmol/l boric acid running buffer (ph 8.5), 45 mmol/l sds, 16 kv voltage, 20 degrees c, and 10.0% (v/v) of acetonitrile. under the optimum conditions, five components could be separated with a good baseline resolution within 17 min. the calibration curves showed good linear relationship over the concentration range of 5 x 10(-4)similar to 0.1 mg/ml for quercetin, rutin, saussurenoside, kaempferol, and 1 x 10(-3) similar to 0.1 mg/ml for oleanolic acid. the average recoveries of the method and rsd were ( 99.2%, 3.2%) for quercetin, (102.1%, 2.1%) for rutin, (99.4%, 1.5%) for saussurenoside, (98.9%, 1.8%) for kaempferol, and (99.0%, 2.9%) for oleanolic acid, respectively. the detection limits (s/n = 3) were 1.1 x 10(-4) mg/ml for quercetin, 2.6 x 10(-4) mg/ml for rutin, 1.8 x 10(-4) mg/ml for saussurenoside, 2.9 x 10(-4) mg/ml for kaempferol, and 6.3 x 10(-4) mg/ml for oleanolic acid, respectively. the method was simple, rapid, and reproducible and could be applied for the determination of quercetin, rutin, saussurenoside, kaempferol, and oleanolic acid in tibetan medicine elsholtzia, and the assay results were satisfactory. |
| 英文摘要 | a high performance capillary electrophoresis method with diode array detector detection for the determination of five bioactive ingredients in tibetan medicine elsholtzia, namely quercetin, rutin, saussurenoside, kaempferol, and oleanolic acid, has been developed. the effects of several factors, such as the acidity, concentration of running buffer, separation voltage, temperature, and sds concentration were investigated. the optimal conditions were 44 mmol/l boric acid running buffer (ph 8.5), 45 mmol/l sds, 16 kv voltage, 20 degrees c, and 10.0% (v/v) of acetonitrile. under the optimum conditions, five components could be separated with a good baseline resolution within 17 min. the calibration curves showed good linear relationship over the concentration range of 5 x 10(-4)similar to 0.1 mg/ml for quercetin, rutin, saussurenoside, kaempferol, and 1 x 10(-3) similar to 0.1 mg/ml for oleanolic acid. the average recoveries of the method and rsd were ( 99.2%, 3.2%) for quercetin, (102.1%, 2.1%) for rutin, (99.4%, 1.5%) for saussurenoside, (98.9%, 1.8%) for kaempferol, and (99.0%, 2.9%) for oleanolic acid, respectively. the detection limits (s/n = 3) were 1.1 x 10(-4) mg/ml for quercetin, 2.6 x 10(-4) mg/ml for rutin, 1.8 x 10(-4) mg/ml for saussurenoside, 2.9 x 10(-4) mg/ml for kaempferol, and 6.3 x 10(-4) mg/ml for oleanolic acid, respectively. the method was simple, rapid, and reproducible and could be applied for the determination of quercetin, rutin, saussurenoside, kaempferol, and oleanolic acid in tibetan medicine elsholtzia, and the assay results were satisfactory. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 生物科学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | inhibiting myocardial apoptosis ; reduce infarct size ; total flavones ; natural-products ; separation ; electrophoresis ; stauntonii ; canines |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250551700008 |
| 公开日期 | 2009-12-07 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1250]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Chenxu,Wang, Lingyun,Zhao, Xianen,et al. Analysis of five pharmacologically active compounds from the Tibetan medicine Elsholtzia with micellar electrokinetic capillary chromatography[J]. journal of liquid chromatography & related technologies,2007,30(20):3069-3083. |
| APA | Ding, Chenxu.,Wang, Lingyun.,Zhao, Xianen.,Li, Yulin.,Wang, Honglun.,...&Suo, Yourui.(2007).Analysis of five pharmacologically active compounds from the Tibetan medicine Elsholtzia with micellar electrokinetic capillary chromatography.journal of liquid chromatography & related technologies,30(20),3069-3083. |
| MLA | Ding, Chenxu,et al."Analysis of five pharmacologically active compounds from the Tibetan medicine Elsholtzia with micellar electrokinetic capillary chromatography".journal of liquid chromatography & related technologies 30.20(2007):3069-3083. |
入库方式： OAI收割
来源：西北高原生物研究所
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