Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS
文献类型：期刊论文
| 作者 | Wang, Li; Xiao, Hongbin; Liang, Xinmiao; Wei, Lixin |
| 刊名 | journal of separation science
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 30期号:10页码:1488-1495 |
| 关键词 | gastrodia elata LC-MS nucleoside phenolic |
| 合作状况 | 其它 |
| 中文摘要 | an hplc-uv-ms method for simultaneous identification of predominant phenolics and minor nucleoside derivatives in gastrodia elata was developed, which was based on their uv and ms characteristics summarized through a series of homemade reference standard experiments. phenolics showed characteristic uv lambda(max) at 267 nm, [m + nh4](+) base peak in positive mode and [m - h](-) base peak in negative mode while nucleosides exhibited uv lambda(max) at 255 nm, [m + h](+), [m - h + 2h(2)o](-) or [m - h + ch3cooh](-). phenolics conjugates mainly underwent the consecutive loss of gastrodin residue (- 268 u) and the combined loss of h2o and co2 from the citric acid unit under negative ms/ms conditions whereas nucleosides simply lost the ribose (- 132 u) under positive ms/ms conditions. according to these characteristics, a special pattern under ms/ms conditions and reported compound data for g. elata in the literature, not only 15 phenolics were identified but also 6 nucleoside derivatives were identified. among these compounds, seven phenolics and three nucleoside derivatives have not been reported yet from g. elata. |
| 英文摘要 | an hplc-uv-ms method for simultaneous identification of predominant phenolics and minor nucleoside derivatives in gastrodia elata was developed, which was based on their uv and ms characteristics summarized through a series of homemade reference standard experiments. phenolics showed characteristic uv lambda(max) at 267 nm, [m + nh4](+) base peak in positive mode and [m - h](-) base peak in negative mode while nucleosides exhibited uv lambda(max) at 255 nm, [m + h](+), [m - h + 2h(2)o](-) or [m - h + ch3cooh](-). phenolics conjugates mainly underwent the consecutive loss of gastrodin residue (- 268 u) and the combined loss of h2o and co2 from the citric acid unit under negative ms/ms conditions whereas nucleosides simply lost the ribose (- 132 u) under positive ms/ms conditions. according to these characteristics, a special pattern under ms/ms conditions and reported compound data for g. elata in the literature, not only 15 phenolics were identified but also 6 nucleoside derivatives were identified. among these compounds, seven phenolics and three nucleoside derivatives have not been reported yet from g. elata. |
| 学科主题 | 生物科学 |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | p-hydroxybenzyl alcohol ; capillary-electrophoresis ; mass-spectrometry ; parishin-b ; blume ; chromatography ; inhibition ; rhizoma |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248240000013 |
| 公开日期 | 2009-12-07 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1262]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Res Ctr Tibetan Med, NW Plateau Inst Biol, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Li,Xiao, Hongbin,Liang, Xinmiao,et al. Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS[J]. journal of separation science,2007,30(10):1488-1495. |
| APA | Wang, Li,Xiao, Hongbin,Liang, Xinmiao,&Wei, Lixin.(2007).Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS.journal of separation science,30(10),1488-1495. |
| MLA | Wang, Li,et al."Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS".journal of separation science 30.10(2007):1488-1495. |
入库方式： OAI收割
来源：西北高原生物研究所
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