Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers
文献类型：期刊论文
| 作者 | Peng, Xiao-Li; Zhao, Chang-Ming; Wu, Gui-Li; Liu, Jian-Quan |
| 刊名 | trees-structure and function
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 21期号:4页码:457-464 |
| 关键词 | Picea likiangensis genetic diversity RAPD morphological differentiation |
| 合作状况 | 其它 |
| 中文摘要 | repeated cycles of retreat and recolonization during the quaternary ice ages are thought to have greatly influenced current species distributions and their genetic diversity. it remains unclear how this climatic oscillation has affected the distribution of genetic diversity between populations of wind-pollinated conifers in the qinghai-tibetan region. in this study, we investigated the within-species genetic diversity and phylogenetic relationships of picea likiangensis, a dominant forest species in this region using polymorphic dna (rapd) markers. our results suggest that this species has high overall genetic diversity, with 85.42% of loci being polymorphic and an average expected heterozygosity (h (e)) of 0.239. however, there were relatively low levels of polymorphism at population levels and the differences between populations were not significant, with percentages of polymorphic bands (ppb) ranging from 46.88 to 69.76%, nei's gene diversity (h (e)) from 0.179 to 0.289 and shannon's indices (hpop) from 0.267 to 0.421. in accordance with our proposed hypothesis, a high level of genetic differentiation among populations was detected based on nei's genetic diversity (g (st) = 0.256) and amova analysis (phi (st) = 0.236). gene flow between populations was found to be limited (nm = 1.4532) and far lower than reported for other conifer species with wide distribution ranges from other regions. no clusters corresponding to three morphological varieties found in the south, north and west, respectively, were detected in either upgma or pco analyses. our results suggest that this species may have had different refugia during the glacial stages in the southern region and that the northern variety may have multiple origins from these different refugia. |
| 英文摘要 | repeated cycles of retreat and recolonization during the quaternary ice ages are thought to have greatly influenced current species distributions and their genetic diversity. it remains unclear how this climatic oscillation has affected the distribution of genetic diversity between populations of wind-pollinated conifers in the qinghai-tibetan region. in this study, we investigated the within-species genetic diversity and phylogenetic relationships of picea likiangensis, a dominant forest species in this region using polymorphic dna (rapd) markers. our results suggest that this species has high overall genetic diversity, with 85.42% of loci being polymorphic and an average expected heterozygosity (h (e)) of 0.239. however, there were relatively low levels of polymorphism at population levels and the differences between populations were not significant, with percentages of polymorphic bands (ppb) ranging from 46.88 to 69.76%, nei's gene diversity (h (e)) from 0.179 to 0.289 and shannon's indices (hpop) from 0.267 to 0.421. in accordance with our proposed hypothesis, a high level of genetic differentiation among populations was detected based on nei's genetic diversity (g (st) = 0.256) and amova analysis (phi (st) = 0.236). gene flow between populations was found to be limited (nm = 1.4532) and far lower than reported for other conifer species with wide distribution ranges from other regions. no clusters corresponding to three morphological varieties found in the south, north and west, respectively, were detected in either upgma or pco analyses. our results suggest that this species may have had different refugia during the glacial stages in the southern region and that the northern variety may have multiple origins from these different refugia. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | forestry |
| 研究领域[WOS] | forestry |
| 关键词[WOS] | south-american conifer ; forest trees ; populations ; diversity ; allozyme ; evolution ; differentiation ; conservation ; cupressaceae ; california |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247659000007 |
| 公开日期 | 2009-12-07 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1265]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Xiao-Li,Zhao, Chang-Ming,Wu, Gui-Li,et al. Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers[J]. trees-structure and function,2007,21(4):457-464. |
| APA | Peng, Xiao-Li,Zhao, Chang-Ming,Wu, Gui-Li,&Liu, Jian-Quan.(2007).Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers.trees-structure and function,21(4),457-464. |
| MLA | Peng, Xiao-Li,et al."Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers".trees-structure and function 21.4(2007):457-464. |
入库方式： OAI收割
来源：西北高原生物研究所
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