Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines
文献类型：期刊论文
| 作者 | You, Jinmao; Ding, Chenxu; Zhu, Fang; Sun, Xuejun; Li, Yulin; Suo, Yourui |
| 刊名 | chromatographia
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 65期号:9-10页码:545-553 |
| 关键词 | column liquid chromatography-mass spectrometry Tibetan herbal medicines phenolic compounds dansyl derivatization |
| 合作状况 | 其它 |
| 中文摘要 | a highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography-mass spectrometry has been developed for identification of natural pharmacologically active phenolic compounds in extracts of lomatogonium rotatum plants (tibetan herbal medicine) obtained by solid-phase extraction. the number of hydroxyl groups on the dansylated phenols was estimated by lc-ms-ms analysis in positive-ion mode. dansyl derivatization of the compounds introduced basic secondary nitrogen into the phenolic core structures and this was readily ionized when acidic hplc mobile phases were used. ms fragmentation of the derivatives generated intense protonated molecular ions of m/z [mh](+) (phenol aglycones were transformed into the corresponding free phenols by cleavage of an aglycone bond). collision-induced dissociation of the protonated molecule generated characteristic product ions of m/z 234 and 171 corresponding to the protonated 5-(dimethylamino)naphthalene sulfoxide and 5 -(dimethylamino) naphthalene moieties, respectively. selected reaction monitoring based on the m/z [mh](+) to 234 and 171 transitions was highly specific for these phenolic compounds. characteristic ions with m/z values of [mh - 234](+), [mh 2 x 234](+), and [mh - 3 x 234](+) were of great importance for estimation of the presence of multihydroxyl groups on the phenolic backbone. |
| 英文摘要 | a highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography-mass spectrometry has been developed for identification of natural pharmacologically active phenolic compounds in extracts of lomatogonium rotatum plants (tibetan herbal medicine) obtained by solid-phase extraction. the number of hydroxyl groups on the dansylated phenols was estimated by lc-ms-ms analysis in positive-ion mode. dansyl derivatization of the compounds introduced basic secondary nitrogen into the phenolic core structures and this was readily ionized when acidic hplc mobile phases were used. ms fragmentation of the derivatives generated intense protonated molecular ions of m/z [mh](+) (phenol aglycones were transformed into the corresponding free phenols by cleavage of an aglycone bond). collision-induced dissociation of the protonated molecule generated characteristic product ions of m/z 234 and 171 corresponding to the protonated 5-(dimethylamino)naphthalene sulfoxide and 5 -(dimethylamino) naphthalene moieties, respectively. selected reaction monitoring based on the m/z [mh](+) to 234 and 171 transitions was highly specific for these phenolic compounds. characteristic ions with m/z values of [mh - 234](+), [mh 2 x 234](+), and [mh - 3 x 234](+) were of great importance for estimation of the presence of multihydroxyl groups on the phenolic backbone. |
| 学科主题 | 生物化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | flavonoids ; fragmentation ; resolution ; extracts ; uv ; ms |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246878900007 |
| 公开日期 | 2009-12-07 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1276]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Ding, Chenxu,Zhu, Fang,et al. Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines[J]. chromatographia,2007,65(9-10):545-553. |
| APA | You, Jinmao,Ding, Chenxu,Zhu, Fang,Sun, Xuejun,Li, Yulin,&Suo, Yourui.(2007).Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines.chromatographia,65(9-10),545-553. |
| MLA | You, Jinmao,et al."Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines".chromatographia 65.9-10(2007):545-553. |
入库方式： OAI收割
来源：西北高原生物研究所
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