Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection
文献类型：期刊论文
| 作者 | You, Jinmao; Zhu, Fang; Zhao, Wenchen; Zhao, Xian'en; Suo, Yourui; Liu, Sujuan |
| 刊名 | european journal of lipid science and technology
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 109期号:3页码:225-236 |
| 关键词 | rape bee pollen fatty acids 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-d]imidazol-1-yl) ethyl-p-toluenesulfonate (ANITS) HPLC fluorescence detection. |
| 合作状况 | 其它 |
| 中文摘要 | a sensitive method for the determination of free fatty acids using 2-(2-(anthracen-10-yl)-1h-naphtho[2,3-dimidazol-1-yl) ethyl-p-toluenesuifonate (anits) as tagging reagent with fluorescence detection has been developed. anits could easily and quickly label fatty acids in the presence of the k2co3 catalyst at 90 degrees c for 40 min in n,n-dimethylformamide solvent. from the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. fatty acid derivatives were separated on a reversed-phase eclipse xdb-c8 column by hplc in conjunction with gradient elution. the corresponding derivatives were identified by post-column apci/ms in positive-ion detection mode. anits-fatty acid derivatives gave an intense molecular ion peak at mlz [m+h](+); with ms/ms analysis, the collision-induced dissociation spectra of m/z [m+h](+) produced the specific fragment ions at mlz [m-345](+) and mlz 345.0 (here, m/z 345 is the core structural moiety of the anits molecule). the fluorescence excitation and emission wavelengths of the derivatives were lambda(ex) = 250 nm and lambda(em) = 512 nm, respectively. linear correlation coefficients for all fatty acid derivatives are > 0.9999. detection limits, at a signal-to-noise ratio of 3 : 1, are 24.76-98.79 fmol for the labeled fatty acids. |
| 英文摘要 | a sensitive method for the determination of free fatty acids using 2-(2-(anthracen-10-yl)-1h-naphtho[2,3-dimidazol-1-yl) ethyl-p-toluenesuifonate (anits) as tagging reagent with fluorescence detection has been developed. anits could easily and quickly label fatty acids in the presence of the k2co3 catalyst at 90 degrees c for 40 min in n,n-dimethylformamide solvent. from the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. fatty acid derivatives were separated on a reversed-phase eclipse xdb-c8 column by hplc in conjunction with gradient elution. the corresponding derivatives were identified by post-column apci/ms in positive-ion detection mode. anits-fatty acid derivatives gave an intense molecular ion peak at mlz [m+h](+); with ms/ms analysis, the collision-induced dissociation spectra of m/z [m+h](+) produced the specific fragment ions at mlz [m-345](+) and mlz 345.0 (here, m/z 345 is the core structural moiety of the anits molecule). the fluorescence excitation and emission wavelengths of the derivatives were lambda(ex) = 250 nm and lambda(em) = 512 nm, respectively. linear correlation coefficients for all fatty acid derivatives are > 0.9999. detection limits, at a signal-to-noise ratio of 3 : 1, are 24.76-98.79 fmol for the labeled fatty acids. |
| 学科主题 | 生物科学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | food science & technology ; nutrition & dietetics |
| 研究领域[WOS] | food science & technology ; nutrition & dietetics |
| 关键词[WOS] | performance liquid-chromatography ; carboxylic-acids ; derivatization reagent ; labeling reagent ; fluorometric detection ; trifluoromethanesulfonate ; 9-anthryldiazomethane ; derivatives ; prostaglandins ; serum |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245200500006 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1287]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Shandong 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Zhu, Fang,Zhao, Wenchen,et al. Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection[J]. european journal of lipid science and technology,2007,109(3):225-236. |
| APA | You, Jinmao,Zhu, Fang,Zhao, Wenchen,Zhao, Xian'en,Suo, Yourui,&Liu, Sujuan.(2007).Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection.european journal of lipid science and technology,109(3),225-236. |
| MLA | You, Jinmao,et al."Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection".european journal of lipid science and technology 109.3(2007):225-236. |
入库方式： OAI收割
来源：西北高原生物研究所
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