Study of a new derivatizing reagent that improves the analysis of amino acids by HPLC with fluorescence detection: application to hydrolyzed rape bee pollen
文献类型：期刊论文
| 作者 | You, Jinmao; Liu, Lingjun; Zhao, Wenchen; Zhao, Xianen; Suo, Yourui; Wang, Honglun; Li, Yulin |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 387期号:8页码:2705-2718 |
| 关键词 | Rape bee pollen amino acids 2-(11H-benzo[alpha]-carbazol-11-yl) ethyl chloroformate (BCEC-Cl) |
| 合作状况 | 其它 |
| 中文摘要 | a simple and sensitive method for evaluating the chemical compositions of protein amino acids, including cystine (cys)(2) and tryptophane (try) has been developed, based on the use of a sensitive labeling reagent 2-(11h-benzo[alpha]-carbazol-11-yl) ethyl chloroformate (bcec-cl) along with fluorescence detection. the chromophore of the 1,2-benzo-3,4-dihydrocarbazole-ethyl chloroformate (bceoc-cl) molecule was replaced with the 2-(11h-benzo[alpha]-carbazol-11-yl) ethyl functional group, yielding the sensitive fluorescence molecule bcec-cl. the new reagent bcec-cl could then be substituted for labeling reagents commonly used in amino acid derivatization. the bcec-amino acid derivatives exhibited very high detection sensitivities, particularly in the cases of (cys)(2) and try, which cannot be determined using traditional labeling reagents such as 9-fluorenyl methylchloroformate (fmoc-cl) and ortho-phthaldialdehyde (opa). the fluorescence detection intensities for the bcec derivatives were compared to those obtained when using fmoc-cl and bceoc-cl as labeling reagents. the ratios i (bcec)/i (bceoc) = 1.17-3.57, i (bcec)/i (fmoc) = 1.13-8.21, and uvbcec/uvbceoc = 1.67-4.90 (where i is the fluorescence intensity and uv is the ultraviolet absorbance). derivative separation was optimized on a hypersil bds c-18 column. the detection limits calculated from 1.0 pmol injections, at a signal-to-noise ratio of 3, ranged from 7.2 fmol for try to 8.4 fmol for (cys)(2). excellent linear responses were observed, with coefficients of > 0.9994. when coupled with high-performance liquid chromatography, the method established here allowed the development of a highly sensitive and specific method for the quantitative analysis of trace levels of amino acids including (cys)(2) and try from bee-collected pollen (bee pollen) samples. |
| 英文摘要 | a simple and sensitive method for evaluating the chemical compositions of protein amino acids, including cystine (cys)(2) and tryptophane (try) has been developed, based on the use of a sensitive labeling reagent 2-(11h-benzo[alpha]-carbazol-11-yl) ethyl chloroformate (bcec-cl) along with fluorescence detection. the chromophore of the 1,2-benzo-3,4-dihydrocarbazole-ethyl chloroformate (bceoc-cl) molecule was replaced with the 2-(11h-benzo[alpha]-carbazol-11-yl) ethyl functional group, yielding the sensitive fluorescence molecule bcec-cl. the new reagent bcec-cl could then be substituted for labeling reagents commonly used in amino acid derivatization. the bcec-amino acid derivatives exhibited very high detection sensitivities, particularly in the cases of (cys)(2) and try, which cannot be determined using traditional labeling reagents such as 9-fluorenyl methylchloroformate (fmoc-cl) and ortho-phthaldialdehyde (opa). the fluorescence detection intensities for the bcec derivatives were compared to those obtained when using fmoc-cl and bceoc-cl as labeling reagents. the ratios i (bcec)/i (bceoc) = 1.17-3.57, i (bcec)/i (fmoc) = 1.13-8.21, and uvbcec/uvbceoc = 1.67-4.90 (where i is the fluorescence intensity and uv is the ultraviolet absorbance). derivative separation was optimized on a hypersil bds c-18 column. the detection limits calculated from 1.0 pmol injections, at a signal-to-noise ratio of 3, ranged from 7.2 fmol for try to 8.4 fmol for (cys)(2). excellent linear responses were observed, with coefficients of > 0.9994. when coupled with high-performance liquid chromatography, the method established here allowed the development of a highly sensitive and specific method for the quantitative analysis of trace levels of amino acids including (cys)(2) and try from bee-collected pollen (bee pollen) samples. |
| 学科主题 | 生物化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; precolumn derivatization ; mass-spectrometry ; 9-fluorenylmethyl chloroformate ; 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate ; gas-chromatography ; biogenic-amines ; ionization ; hydroxyproline ; identification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245123600014 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1288]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Dept Chem, Shandong 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Liu, Lingjun,Zhao, Wenchen,et al. Study of a new derivatizing reagent that improves the analysis of amino acids by HPLC with fluorescence detection: application to hydrolyzed rape bee pollen[J]. analytical and bioanalytical chemistry,2007,387(8):2705-2718. |
| APA | You, Jinmao.,Liu, Lingjun.,Zhao, Wenchen.,Zhao, Xianen.,Suo, Yourui.,...&Li, Yulin.(2007).Study of a new derivatizing reagent that improves the analysis of amino acids by HPLC with fluorescence detection: application to hydrolyzed rape bee pollen.analytical and bioanalytical chemistry,387(8),2705-2718. |
| MLA | You, Jinmao,et al."Study of a new derivatizing reagent that improves the analysis of amino acids by HPLC with fluorescence detection: application to hydrolyzed rape bee pollen".analytical and bioanalytical chemistry 387.8(2007):2705-2718. |
入库方式： OAI收割
来源：西北高原生物研究所
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