Effects of azadirachtin on hemolymph protein expression in Ostrinia furnacalis (Lepidoptera : Crambidae)
文献类型：期刊论文
| 作者 | Huang, Zhiwei; Shi, Ping; Chen, Guichen; Du, Jiawei |
| 刊名 | annals of the entomological society of america
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 100期号:2页码:245-250 |
| 关键词 | azadirachtin Ostrinia furnacalis hemolymph protein expression lipid metabolism |
| 合作状况 | 其它 |
| 中文摘要 | the botanical insecticide azadirachtin affects a variety of biological processes. our early work indicated that protein level and type are significantly influenced by azadirachtin in pupae of osttiniafumacalis (guenee) (lepidoptera: crambidae) because a correlation exists between protein content and azadiraebtin concentration. by use of proteomic techniques, we analyzed changes in hemolymph protein expression of 48-h-old pupae in o. furnacalis induced by azadirachtin treatment. after feeding by third instars on an artificial diet containing 10 ppm azadirachtin until pupation, 48-b-old pupae were collected, and hemolymph protein samples were prepared. they were separated by two-dimensional polyacrylamide gel electrophoresis, and six proteins were significantly affected by azadiracbtin treatment compared with an untreated control. two of these proteins were identified by database searching with peptide mass fingerprinting by using matrix-assisted laser desorption/ time-of-flight mass spectrometry after in-gel trypsin digestion. they belong to the insect apolipophorin-iii and phospboribosyltransferase family, respectively. these two proteins may function on lipid metabolism in insect hemolymph. furthermore, fat body is the center of synthesis and secretion of hemolymph proteins. we suggest that the azadirachtin exerts its insecticidal effects on the fat body of o. furnacalis by interfering with protein expression related to hemolymph lipid metabolism. |
| 英文摘要 | the botanical insecticide azadirachtin affects a variety of biological processes. our early work indicated that protein level and type are significantly influenced by azadirachtin in pupae of osttiniafumacalis (guenee) (lepidoptera: crambidae) because a correlation exists between protein content and azadiraebtin concentration. by use of proteomic techniques, we analyzed changes in hemolymph protein expression of 48-h-old pupae in o. furnacalis induced by azadirachtin treatment. after feeding by third instars on an artificial diet containing 10 ppm azadirachtin until pupation, 48-b-old pupae were collected, and hemolymph protein samples were prepared. they were separated by two-dimensional polyacrylamide gel electrophoresis, and six proteins were significantly affected by azadiracbtin treatment compared with an untreated control. two of these proteins were identified by database searching with peptide mass fingerprinting by using matrix-assisted laser desorption/ time-of-flight mass spectrometry after in-gel trypsin digestion. they belong to the insect apolipophorin-iii and phospboribosyltransferase family, respectively. these two proteins may function on lipid metabolism in insect hemolymph. furthermore, fat body is the center of synthesis and secretion of hemolymph proteins. we suggest that the azadirachtin exerts its insecticidal effects on the fat body of o. furnacalis by interfering with protein expression related to hemolymph lipid metabolism. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 昆虫学 |
| 类目[WOS] | entomology |
| 研究领域[WOS] | entomology |
| 关键词[WOS] | medinalis guenee ; rice leaffolder ; neem limonoids ; metabolism ; insecticide |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244762800016 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1294]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Zhiwei,Shi, Ping,Chen, Guichen,et al. Effects of azadirachtin on hemolymph protein expression in Ostrinia furnacalis (Lepidoptera : Crambidae)[J]. annals of the entomological society of america,2007,100(2):245-250. |
| APA | Huang, Zhiwei,Shi, Ping,Chen, Guichen,&Du, Jiawei.(2007).Effects of azadirachtin on hemolymph protein expression in Ostrinia furnacalis (Lepidoptera : Crambidae).annals of the entomological society of america,100(2),245-250. |
| MLA | Huang, Zhiwei,et al."Effects of azadirachtin on hemolymph protein expression in Ostrinia furnacalis (Lepidoptera : Crambidae)".annals of the entomological society of america 100.2(2007):245-250. |
入库方式： OAI收割
来源：西北高原生物研究所
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