Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai-Tibet Plateau
文献类型：期刊论文
| 作者 | Zhang, Defang; Chen, Shilong; Chen, Shengyun; Zhang, Dejun; Gao, Qingbo |
| 刊名 | biochemical genetics
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 45期号:1-2页码:33-50 |
| 关键词 | Swertia przewalskii genetic variation RAPD ISSR endemic plant Qinghai-Tibet Plateau |
| 合作状况 | 其它 |
| 中文摘要 | swertia przewalskii pissjauk. (gentianaceae) is a critically endangered and endemic plant of the qinghai-tibet plateau in china. rapd and issr analyses were carried out on a total of 63 individuals to assess the extent of genetic variation in the remaining three populations. percentage of polymorphic bands was 94% (156 bands) for rapd and 96% (222 bands) for issr. a pairwise distance measure calculated from the rapd and issr data was used as input for analysis of molecular variance (amova). amova indicated that a high proportion of the total genetic variation (52% for rapd and 56% for issr) was found among populations; pairwise phi(st) comparisons showed that the three populations examined were significantly different (p < 0.001). significant genetic differentiation was found based on different measures (amova and hickory theta(b)) in s. przewalskii (0.52 on rapd and 0.56 on issr; 0.46 on rapd and 0.45 on issr). the differentiation of the populations corresponded to low average gene flow (0.28 based on rapd and 0.31 based on issr), whereas genetic distance-based clustering and coalescent-based assignment analyses revealed significant genetic isolation among populations. our results indicate that genetic diversity is independent of population size. we conclude that although sexual reproduction and gene flow between populations of s. przewalskii are very limited, they have preserved high levels of genetic diversity. the main factors responsible for the high level of difference among populations are the isolation and recent fragmentation under human disturbance. |
| 英文摘要 | swertia przewalskii pissjauk. (gentianaceae) is a critically endangered and endemic plant of the qinghai-tibet plateau in china. rapd and issr analyses were carried out on a total of 63 individuals to assess the extent of genetic variation in the remaining three populations. percentage of polymorphic bands was 94% (156 bands) for rapd and 96% (222 bands) for issr. a pairwise distance measure calculated from the rapd and issr data was used as input for analysis of molecular variance (amova). amova indicated that a high proportion of the total genetic variation (52% for rapd and 56% for issr) was found among populations; pairwise phi(st) comparisons showed that the three populations examined were significantly different (p < 0.001). significant genetic differentiation was found based on different measures (amova and hickory theta(b)) in s. przewalskii (0.52 on rapd and 0.56 on issr; 0.46 on rapd and 0.45 on issr). the differentiation of the populations corresponded to low average gene flow (0.28 based on rapd and 0.31 based on issr), whereas genetic distance-based clustering and coalescent-based assignment analyses revealed significant genetic isolation among populations. our results indicate that genetic diversity is independent of population size. we conclude that although sexual reproduction and gene flow between populations of s. przewalskii are very limited, they have preserved high levels of genetic diversity. the main factors responsible for the high level of difference among populations are the isolation and recent fragmentation under human disturbance. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 生物化学 |
| 类目[WOS] | biochemistry & molecular biology ; genetics & heredity |
| 研究领域[WOS] | biochemistry & molecular biology ; genetics & heredity |
| 关键词[WOS] | rapd markers ; natural-populations ; clonal diversity ; issr markers ; plant ; differentiation ; china ; aflp ; rare ; size |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244191000004 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1298]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Lab Qinghai Tibet Biol Evolut & Adaptat, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Defang,Chen, Shilong,Chen, Shengyun,et al. Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai-Tibet Plateau[J]. biochemical genetics,2007,45(1-2):33-50. |
| APA | Zhang, Defang,Chen, Shilong,Chen, Shengyun,Zhang, Dejun,&Gao, Qingbo.(2007).Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai-Tibet Plateau.biochemical genetics,45(1-2),33-50. |
| MLA | Zhang, Defang,et al."Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai-Tibet Plateau".biochemical genetics 45.1-2(2007):33-50. |
入库方式： OAI收割
来源：西北高原生物研究所
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