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Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE
文献类型：期刊论文
| 作者 | Wang, Honglun; Wang, Xiaoyan; You, Jinmao; Suo, Yourui; Zhao, Xianen; Li, Yulin; Ding, Chenxu |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 30期号:3页码:363-376 |
| 关键词 | allantoin quercetin 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-car-boxylicb acid Nitraria tangutorum Bobr high performance liquid chromatography, atmospheric pressure chemical ionization mass spectrometry capillary electrophoresis |
| 合作状况 | 其它 |
| 中文摘要 | this paper describes the simultaneous determination of allantoin, quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (mtcca) in nitraria tangutorum bobr seed by hplc-apci-ms and ce (capillary electrophoresis) methods. the final optimized chromatographic conditions were investigated in a reversed-phase eclipse xdb-c8 column (150 x 4.6 mm, 5 mu m). a seventeen-minute gradient elution, (a: aqueous acetonitrile 20% (v/v); b: aqueous acetonitrile 60% (v/v); c: pure acetonitrile 100%) at a flow rate of 1.0 ml/min was selected for the separation of three natural products with diode array detection (dad) at 220 nm. a ce experiment was carried out in a fused silica capillary with 32 mmol/l boric acid (ph 10), 32 mmol/l sds and acetonitrile (10.0%, v/v). the applied potential and temperature was, respectively, set at 19 kv and 25 degrees c. after development, the validation was performed in parallel for hplc and ce, with the same standards and sample to avoid differences due to the manipulation. the validation parameters of both techniques were adequate for the intended purpose. |
| 英文摘要 | this paper describes the simultaneous determination of allantoin, quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (mtcca) in nitraria tangutorum bobr seed by hplc-apci-ms and ce (capillary electrophoresis) methods. the final optimized chromatographic conditions were investigated in a reversed-phase eclipse xdb-c8 column (150 x 4.6 mm, 5 mu m). a seventeen-minute gradient elution, (a: aqueous acetonitrile 20% (v/v); b: aqueous acetonitrile 60% (v/v); c: pure acetonitrile 100%) at a flow rate of 1.0 ml/min was selected for the separation of three natural products with diode array detection (dad) at 220 nm. a ce experiment was carried out in a fused silica capillary with 32 mmol/l boric acid (ph 10), 32 mmol/l sds and acetonitrile (10.0%, v/v). the applied potential and temperature was, respectively, set at 19 kv and 25 degrees c. after development, the validation was performed in parallel for hplc and ce, with the same standards and sample to avoid differences due to the manipulation. the validation parameters of both techniques were adequate for the intended purpose. |
| 学科主题 | 生物化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243121900004 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1302]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Platueau Inst Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Honglun,Wang, Xiaoyan,You, Jinmao,et al. Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE[J]. journal of liquid chromatography & related technologies,2007,30(3):363-376. |
| APA | Wang, Honglun.,Wang, Xiaoyan.,You, Jinmao.,Suo, Yourui.,Zhao, Xianen.,...&Ding, Chenxu.(2007).Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE.journal of liquid chromatography & related technologies,30(3),363-376. |
| MLA | Wang, Honglun,et al."Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE".journal of liquid chromatography & related technologies 30.3(2007):363-376. |
入库方式： OAI收割
来源：西北高原生物研究所
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