Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice
文献类型：期刊论文
| 作者 | Chen, Xue-Qun; Wang, Shi-Jun; Du, Ji-Zeng; Chen, Xiao-Cheng |
| 刊名 | american journal of physiology-regulatory integrative and comparative physiology
 |
| 出版日期 | 2007 |
| 卷号 | 292期号:1页码:r516-r526 |
| 关键词 | hypoxia-inducible factor insulin-like growth factor lactate dehydrogenase-A Tibetan mammal cobalt chloride hypoxia |
| 合作状况 | 其它 |
| 中文摘要 | ochotona curzoniae and microtus oeconomus are the native mammals living on the qinghai-tibetanplateau of china. the molecular mechanisms of their acclimatization to the plateau-hypoxia remain unclear. expressions of hepatic hypoxia-inducible factor (hif)-1 alpha, insulin-like growth factor-i (igf-i)/igf binding protein (bp)-1(igfbp-1; including genes), and key metabolic enzymatic genes [lactate dehydrogenase (ldh)-a/isocitrate dehydrogenase (icd)] are compared in qinghai-tibetan- plateau mammals andsea- level mice after injection of cocl2 (20, 40, or 60 mg/ kg) and normobaric hypoxia (16.0% o-2, 10.8% o-2, and 8.0% o-2) for 6 h, tested by histochemistry, western blot analysis, elisa, and rt-pcr. major results are cocl2 markedly increased 1) hif-1 alpha only in mice, 2) hepatic and circulatory igf-i in m. oeconomus, 3) hepatic igfbp-1 in mice and o. curzoniae, and 4) ldh-a but reduced icd mrna in mice (cocl2 20 mg/kg) but were unchanged in the tibetan mammals. normobaric hypoxia markedly 1) increased hif-1 alpha and ldh-a mrna in mice and m. oeconomus (8.0% o-2) not in o. curzoniae, and 2) reduced icd mrna in mice and m. oeconomus (8.0% o-2) not in o. curzoniae. results suggest that 1) hif-1 alpha responsiveness to hypoxia is distinct in lowland mice and plateau mammals, reflecting a diverse tolerance of the three species to hypoxia; 2) cocl2 induces diversities in hif-1, igf-i/igfbp-1 protein or genes in mice, m. oeconomus, and o. curzoniae. in contrast, hif-1 mediates igfbp-1 transcription only in mice and in m. oeconomus (subjected to severe hypoxia); 3) differences in igf-i/igfbp-1 expressions induced by cocl2 reflect significant diversities in hormone regulation and cell protection from damage; and 4) activation of anaerobic glycolysis and reduction of krebs cycle represents strategies of lowland-animals vs. the stable metabolic homeostasis of plateau- acclimatized mammals. |
| 英文摘要 | ochotona curzoniae and microtus oeconomus are the native mammals living on the qinghai-tibetanplateau of china. the molecular mechanisms of their acclimatization to the plateau-hypoxia remain unclear. expressions of hepatic hypoxia-inducible factor (hif)-1 alpha, insulin-like growth factor-i (igf-i)/igf binding protein (bp)-1(igfbp-1; including genes), and key metabolic enzymatic genes [lactate dehydrogenase (ldh)-a/isocitrate dehydrogenase (icd)] are compared in qinghai-tibetan- plateau mammals andsea- level mice after injection of cocl2 (20, 40, or 60 mg/ kg) and normobaric hypoxia (16.0% o-2, 10.8% o-2, and 8.0% o-2) for 6 h, tested by histochemistry, western blot analysis, elisa, and rt-pcr. major results are cocl2 markedly increased 1) hif-1 alpha only in mice, 2) hepatic and circulatory igf-i in m. oeconomus, 3) hepatic igfbp-1 in mice and o. curzoniae, and 4) ldh-a but reduced icd mrna in mice (cocl2 20 mg/kg) but were unchanged in the tibetan mammals. normobaric hypoxia markedly 1) increased hif-1 alpha and ldh-a mrna in mice and m. oeconomus (8.0% o-2) not in o. curzoniae, and 2) reduced icd mrna in mice and m. oeconomus (8.0% o-2) not in o. curzoniae. results suggest that 1) hif-1 alpha responsiveness to hypoxia is distinct in lowland mice and plateau mammals, reflecting a diverse tolerance of the three species to hypoxia; 2) cocl2 induces diversities in hif-1, igf-i/igfbp-1 protein or genes in mice, m. oeconomus, and o. curzoniae. in contrast, hif-1 mediates igfbp-1 transcription only in mice and in m. oeconomus (subjected to severe hypoxia); 3) differences in igf-i/igfbp-1 expressions induced by cocl2 reflect significant diversities in hormone regulation and cell protection from damage; and 4) activation of anaerobic glycolysis and reduction of krebs cycle represents strategies of lowland-animals vs. the stable metabolic homeostasis of plateau- acclimatized mammals. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 生理学 |
| 类目[WOS] | physiology |
| 研究领域[WOS] | physiology |
| 关键词[WOS] | growth-factor-i ; factor-binding protein-1 ; induced gene-expression ; inducible factor-i ; igf-i ; rat-brain ; skeletal-muscle ; liver-injury ; hepg2 cells ; hypoxia |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243171900060 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1304]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Zhejiang Univ, Coll Life Sci, Div Neurobiol & Physiol, Hangzhou 310027, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xue-Qun,Wang, Shi-Jun,Du, Ji-Zeng,et al. Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice[J]. american journal of physiology-regulatory integrative and comparative physiology,2007,292(1):r516-r526. |
| APA | Chen, Xue-Qun,Wang, Shi-Jun,Du, Ji-Zeng,&Chen, Xiao-Cheng.(2007).Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice.american journal of physiology-regulatory integrative and comparative physiology,292(1),r516-r526. |
| MLA | Chen, Xue-Qun,et al."Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice".american journal of physiology-regulatory integrative and comparative physiology 292.1(2007):r516-r526. |
入库方式： OAI收割
来源：西北高原生物研究所
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