Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii
文献类型：期刊论文
| 作者 | Xue, Chun-Ying; Li, De-Zhu; Lu, Jin-Mei; Yang, Jun-Bo; Liu, Jian-Quan |
| 刊名 | planta medica
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 72期号:13页码:1223-1226 |
| 关键词 | Tibetan folk medicine Swertia mussotii Gentianaceae DNA sequences allele-specific diagnostic PCR authentication |
| 合作状况 | 其它 |
| 中文摘要 | swertia mussotii is an important species in tibetan folk medicine. however, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. we show here that the chloroplast (cp) dna rpl16 intron has limited utility for differentiating s. mussotii from closely related species, since the cpdna rpl16 sequences are identical in s. mussotii and two other species of swertia. however, the rdna internal transcribed spacer (its) sequences differ significantly between s. mussotii and all of 13 tested potential adulterants. thus, the its region provides a robust molecular marker for differentiating the medicinal s. mussotii from related adulterants. therefore, a pair of allele-specific diagnostic primers based on the divergent its region was designed to distinguish s. mussotii from the other species. authentication by allele-specific diagnostic pcr using these primers is convenient, effective and both simpler and less time-consuming than sequencing the its region. |
| 英文摘要 | swertia mussotii is an important species in tibetan folk medicine. however, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. we show here that the chloroplast (cp) dna rpl16 intron has limited utility for differentiating s. mussotii from closely related species, since the cpdna rpl16 sequences are identical in s. mussotii and two other species of swertia. however, the rdna internal transcribed spacer (its) sequences differ significantly between s. mussotii and all of 13 tested potential adulterants. thus, the its region provides a robust molecular marker for differentiating the medicinal s. mussotii from related adulterants. therefore, a pair of allele-specific diagnostic primers based on the divergent its region was designed to distinguish s. mussotii from the other species. authentication by allele-specific diagnostic pcr using these primers is convenient, effective and both simpler and less time-consuming than sequencing the its region. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 植物学 |
| 类目[WOS] | plant sciences ; chemistry, medicinal ; pharmacology & pharmacy |
| 研究领域[WOS] | plant sciences ; pharmacology & pharmacy |
| 关键词[WOS] | dendrobium-officinale ; ribosomal dna ; nuclear ; region ; spacer |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241876600009 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1307]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Lab Plant Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Chun-Ying,Li, De-Zhu,Lu, Jin-Mei,et al. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii[J]. planta medica,2006,72(13):1223-1226. |
| APA | Xue, Chun-Ying,Li, De-Zhu,Lu, Jin-Mei,Yang, Jun-Bo,&Liu, Jian-Quan.(2006).Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii.planta medica,72(13),1223-1226. |
| MLA | Xue, Chun-Ying,et al."Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii".planta medica 72.13(2006):1223-1226. |
入库方式： OAI收割
来源：西北高原生物研究所
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