HPLC-APCI-MS determination of free fatty acids in Tibet folk medicine Lomatogonium rotatum with fluorescence detection and mass spectrometric identification
文献类型：期刊论文
| 作者 | li, Yun Li; Zhao, Xian'en; Ding, Chenxu; Wang, Honglun; Suo, Yourui; Chen, Guichen; You, Jinmao |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2006 |
| 卷号 | 29期号:18页码:2741-2751 |
| 关键词 | HPLC-APCI-MS FFAs AETS fluorescence detection derivatization Lomatogonium rotatum |
| 合作状况 | 其它 |
| 中文摘要 | a simple and sensitive method for the determination of free fatty acids (ffas) using acridone-9-ethyl-p-toluenesulfonate (aets) as a fluorescence derivatization reagent by high performance liquid chromatography (hplc) has been developed. free fatty acid derivatives were separated on an eclipse xdb-c-8 column with a good baseline resolution and detected with the fluorescence of which excitation and emission wavelengths of derivatives were set at lambda(ex) 404 and lambda(em) 440 nm, respectively. identification of 19 fatty acid derivatives was carried out by online post-column mass spectrometry with an atmospheric pressure chemical ionization (apci) source under positive-ion detection mode. nineteen ffas from the extract of lomatogonium rotatum are sensitively determined. the results indicate that the plant lomatogonium rotatum is enriched with an abundance of ffas and ffas of higher contents, which mainly focus on even carbon atoms, c-14, c-16, and c-18. the validation of the method including linearity, repeatability, and detection limits was examined. most linear correlation coefficients for fatty acid derivatives are > 0.9989, and detection limits (at signal-to-noise of 3: 1) are 12.3-43.7 fmol. the relative standard deviations (rsds) of the peak areas and retention times for 19 ffas standards are < 2.24% and 0.45%, respectively. the established method is rapid and reproducible for the separation determination of ffas from the extract of lomatogonium rotatum with satisfactory results. |
| 英文摘要 | a simple and sensitive method for the determination of free fatty acids (ffas) using acridone-9-ethyl-p-toluenesulfonate (aets) as a fluorescence derivatization reagent by high performance liquid chromatography (hplc) has been developed. free fatty acid derivatives were separated on an eclipse xdb-c-8 column with a good baseline resolution and detected with the fluorescence of which excitation and emission wavelengths of derivatives were set at lambda(ex) 404 and lambda(em) 440 nm, respectively. identification of 19 fatty acid derivatives was carried out by online post-column mass spectrometry with an atmospheric pressure chemical ionization (apci) source under positive-ion detection mode. nineteen ffas from the extract of lomatogonium rotatum are sensitively determined. the results indicate that the plant lomatogonium rotatum is enriched with an abundance of ffas and ffas of higher contents, which mainly focus on even carbon atoms, c-14, c-16, and c-18. the validation of the method including linearity, repeatability, and detection limits was examined. most linear correlation coefficients for fatty acid derivatives are > 0.9989, and detection limits (at signal-to-noise of 3: 1) are 12.3-43.7 fmol. the relative standard deviations (rsds) of the peak areas and retention times for 19 ffas standards are < 2.24% and 0.45%, respectively. the established method is rapid and reproducible for the separation determination of ffas from the extract of lomatogonium rotatum with satisfactory results. |
| 学科主题 | 生物化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; oil |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240491000011 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1318]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Grad Univ, Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 3.Qufu Normal Univ, Dept Chem, Qufu, Peoples R China |
| 推荐引用方式 GB/T 7714 | li, Yun Li,Zhao, Xian'en,Ding, Chenxu,et al. HPLC-APCI-MS determination of free fatty acids in Tibet folk medicine Lomatogonium rotatum with fluorescence detection and mass spectrometric identification[J]. journal of liquid chromatography & related technologies,2006,29(18):2741-2751. |
| APA | li, Yun Li.,Zhao, Xian'en.,Ding, Chenxu.,Wang, Honglun.,Suo, Yourui.,...&You, Jinmao.(2006).HPLC-APCI-MS determination of free fatty acids in Tibet folk medicine Lomatogonium rotatum with fluorescence detection and mass spectrometric identification.journal of liquid chromatography & related technologies,29(18),2741-2751. |
| MLA | li, Yun Li,et al."HPLC-APCI-MS determination of free fatty acids in Tibet folk medicine Lomatogonium rotatum with fluorescence detection and mass spectrometric identification".journal of liquid chromatography & related technologies 29.18(2006):2741-2751. |
入库方式： OAI收割
来源：西北高原生物研究所
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