Leptin cDNA cloning and its mRNA expression in plateau pikas (Ochotona curzoniae) from different altitudes on Qinghai-Tibet Plateau
文献类型：期刊论文
| 作者 | Yang, J; Zhao, XQ; Guo, SC; Li, HG; Qi, DL; Wang, DP; Cao, JH |
| 刊名 | biochemical and biophysical research communications
 |
| 出版日期 | 2006-07-14 |
| 卷号 | 345期号:4页码:1405-1413 |
| 关键词 | leptin cDNA cloning Plateau pika mRNA expression in different altitudes |
| 合作状况 | 其它 |
| 中文摘要 | leptin, an adipocyte-derived hormone, plays all important role in body energy homeostasis. plateau pika (ochotona curzoniae), an endemic and keystone species living only at 3000-5000 in above sea level oil qinghai-tibet plateau, is a typically high hypoxia and low temperature tolerant mammal with high resting metabolic rate (rmr), non-shivering thermogenesis (nst), and high ratio of oxygen utilization to cope with harsh plateau environment. to explore the molecular mechanism of ecological acclimation in plateau pika, we first cloned pika leptin cdna and compared its mrna expression in different altitudes (3200 and 3900 in) using real-time rtpcr (taqman probe) technology. the full-length pika leptin cdna was 3015 with 504 bp open-reading frame encoding the precursor peptide of 167 amino acids including 21 residues of signal peptide. pika leptin was 70-72% homologous to that of other species and was of similarly structural characteristics with other species. the pika-specific genetic diversity in leptin sequence occurred at twenty sites. with the increase in attitude, there were larger fat store and high level of ob gene expression in plateau pika. our results indicated that leptin is sensitive to cold and hypoxia plateau environment and may play one of important roles in pika's ecological adaptation to harsh plateau environment. (c) 2006 elsevier inc. all rights reserved. |
| 英文摘要 | leptin, an adipocyte-derived hormone, plays all important role in body energy homeostasis. plateau pika (ochotona curzoniae), an endemic and keystone species living only at 3000-5000 in above sea level oil qinghai-tibet plateau, is a typically high hypoxia and low temperature tolerant mammal with high resting metabolic rate (rmr), non-shivering thermogenesis (nst), and high ratio of oxygen utilization to cope with harsh plateau environment. to explore the molecular mechanism of ecological acclimation in plateau pika, we first cloned pika leptin cdna and compared its mrna expression in different altitudes (3200 and 3900 in) using real-time rtpcr (taqman probe) technology. the full-length pika leptin cdna was 3015 with 504 bp open-reading frame encoding the precursor peptide of 167 amino acids including 21 residues of signal peptide. pika leptin was 70-72% homologous to that of other species and was of similarly structural characteristics with other species. the pika-specific genetic diversity in leptin sequence occurred at twenty sites. with the increase in attitude, there were larger fat store and high level of ob gene expression in plateau pika. our results indicated that leptin is sensitive to cold and hypoxia plateau environment and may play one of important roles in pika's ecological adaptation to harsh plateau environment. (c) 2006 elsevier inc. all rights reserved. |
| 学科主题 | 生物化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biophysics |
| 研究领域[WOS] | biochemistry & molecular biology ; biophysics |
| 关键词[WOS] | obese gene-expression ; adipose-tissue ; plasma leptin ; ob/ob mice ; children ; gender ; cells |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238406200018 |
| 公开日期 | 2009-12-08 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1328]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, J,Zhao, XQ,Guo, SC,et al. Leptin cDNA cloning and its mRNA expression in plateau pikas (Ochotona curzoniae) from different altitudes on Qinghai-Tibet Plateau[J]. biochemical and biophysical research communications,2006,345(4):1405-1413. |
| APA | Yang, J.,Zhao, XQ.,Guo, SC.,Li, HG.,Qi, DL.,...&Cao, JH.(2006).Leptin cDNA cloning and its mRNA expression in plateau pikas (Ochotona curzoniae) from different altitudes on Qinghai-Tibet Plateau.biochemical and biophysical research communications,345(4),1405-1413. |
| MLA | Yang, J,et al."Leptin cDNA cloning and its mRNA expression in plateau pikas (Ochotona curzoniae) from different altitudes on Qinghai-Tibet Plateau".biochemical and biophysical research communications 345.4(2006):1405-1413. |
入库方式： OAI收割
来源：西北高原生物研究所
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