The effect of mechanical tension on DNA polymerase activity studied with a two-state model
文献类型：期刊论文
| 作者 | Shu, Yaogen; Shi, Hualin ; Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China
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| 刊名 | MODERN PHYSICS LETTERS B
 |
| 出版日期 | 2007 |
| 卷号 | 21期号:17页码:1097-1105 |
| 关键词 | Single-stranded-dna Replication Fidelity Exonuclease Complex |
| ISSN号 | 0217-9849 |
| 英文摘要 | Single-molecule experiments revealed that the replication rate of T7 DNA polymerase has a peak value at tension of about 5.5 pN. However, the mechanism leading to this peak has only been partially investigated. Here, we present a two-state model to investigate the effect of mechanical tension on DNA polymerase activity. The model consists of polymerase and exonuclease. The polymerase's kinetic pathway has been simplified into Michaelis-Menten form with two steps. One is the conformational change from "open" to "close". The other is the base-stacking. The results are in good agreement with experimental observations. We also predict that the tension on template is beneficial to the fidelity of DNA replication. |
| 学科主题 | Physics |
| URL标识 | 查看原文 |
| WOS记录号 | WOS:000251911900006 |
| 公开日期 | 2012-08-02 |
| 源URL | [http://ir.itp.ac.cn/handle/311006/5693]  |
| 专题 | 理论物理研究所_理论物理所1978-2010年知识产出 |
| 通讯作者 | Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shu, Yaogen,Shi, Hualin,Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China. The effect of mechanical tension on DNA polymerase activity studied with a two-state model[J]. MODERN PHYSICS LETTERS B,2007,21(17):1097-1105. |
| APA | Shu, Yaogen,Shi, Hualin,&Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China.(2007).The effect of mechanical tension on DNA polymerase activity studied with a two-state model.MODERN PHYSICS LETTERS B,21(17),1097-1105. |
| MLA | Shu, Yaogen,et al."The effect of mechanical tension on DNA polymerase activity studied with a two-state model".MODERN PHYSICS LETTERS B 21.17(2007):1097-1105. |
入库方式： OAI收割
来源：理论物理研究所
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