Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers
文献类型：期刊论文
| 作者 | Xia, T; Chen, SL; Chen, SY; Ge, XJ |
| 刊名 | biochemical genetics
 |
| 出版日期 | 2005-04-01 |
| 卷号 | 43期号:3-4页码:87-101 |
| 关键词 | Rhodiola alsia ISSRs genetic variation population structure Qinghai-Tibet Plateau |
| 合作状况 | 其它 |
| 中文摘要 | genetic variation of 10 rhodiola alsia ( crassulaceae) populations from the qinghai - tibet plateau of china was investigated using intersimple sequence repeat (issr) markers. r. alsia is an endemic species of the qinghai - tibet plateau. of the 100 primers screened, 13 were highly polymorphic. using these primers, 140 discernible dna fragments were generated with 112 (80%) being polymorphic, indicating pronounced genetic variation at the species level. also there were high levels of polymorphism at the population level with the percentage of polymorphic bands (ppb) ranging from 63.4 to 88.6%. analysis of molecular variance (amova) showed that the genetic variation was mainly found among populations (70.3%) and variance within populations was 29.7%. the main factors responsible for the high level of differentiation among populations are probably the isolation from other populations and clonal propagation of this species. occasional sexual reproduction might occur in order to maintain high levels of variation within populations. environmental conditions could also influence population genetic structure as they occur in severe habitats. the strong genetic differentiation among populations in our study indicates that the conservation of genetic variability in r. alsia requires maintenance of as many populations as possible. |
| 英文摘要 | genetic variation of 10 rhodiola alsia ( crassulaceae) populations from the qinghai - tibet plateau of china was investigated using intersimple sequence repeat (issr) markers. r. alsia is an endemic species of the qinghai - tibet plateau. of the 100 primers screened, 13 were highly polymorphic. using these primers, 140 discernible dna fragments were generated with 112 (80%) being polymorphic, indicating pronounced genetic variation at the species level. also there were high levels of polymorphism at the population level with the percentage of polymorphic bands (ppb) ranging from 63.4 to 88.6%. analysis of molecular variance (amova) showed that the genetic variation was mainly found among populations (70.3%) and variance within populations was 29.7%. the main factors responsible for the high level of differentiation among populations are probably the isolation from other populations and clonal propagation of this species. occasional sexual reproduction might occur in order to maintain high levels of variation within populations. environmental conditions could also influence population genetic structure as they occur in severe habitats. the strong genetic differentiation among populations in our study indicates that the conservation of genetic variability in r. alsia requires maintenance of as many populations as possible. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 生物化学 |
| 类目[WOS] | biochemistry & molecular biology ; genetics & heredity |
| 研究领域[WOS] | biochemistry & molecular biology ; genetics & heredity |
| 关键词[WOS] | sequence repeat polymorphisms ; rapd variation ; conservation genetics ; natural-populations ; clonal diversity ; endangered plant ; size ; differentiation ; consequences ; dna |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227908200001 |
| 公开日期 | 2009-12-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1378]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Lab Qinghai Tibet Biol Evolut & Adaptat, Xining, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, S China Inst Bot, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, T,Chen, SL,Chen, SY,et al. Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers[J]. biochemical genetics,2005,43(3-4):87-101. |
| APA | Xia, T,Chen, SL,Chen, SY,&Ge, XJ.(2005).Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers.biochemical genetics,43(3-4),87-101. |
| MLA | Xia, T,et al."Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers".biochemical genetics 43.3-4(2005):87-101. |
入库方式： OAI收割
来源：西北高原生物研究所
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