Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis
文献类型：期刊论文
| 作者 | Suo, YR; Wang, HL; Li, YL; You, JM; Wang, HQ |
| 刊名 | chromatographia
 |
| 出版日期 | 2004-11-01 |
| 卷号 | 60期号:9-10页码:589-595 |
| 关键词 | capillary electrophoresis pharmacologically active compounds Rhodiola crenucata Rhodiola quadrifida |
| 合作状况 | 其它 |
| 中文摘要 | a rapid capillary electrophoresis method for the separation of five natural pharmacologically active compounds from extracted rhodiola, namely salidroside, tyrosol, rhodionin, gallic acid and ethyl gallate has been developed. the separation of five natural pharmacologically active compounds was carried out in a fused-silica capillary with 14 mm boric acid, 30 mm sds and 2.5% acetonitrile, adjusted to ph 10.7 with naoh. applied potential was 21 kv. the temperature of the capillary was maintained at 25 degreesc by the instrument thermostating system, with the correlation coefficients of 0.9805-0.9989 for migration time, and relative standards of < 3.52% for peak areas. the established method is rapid and reproducible for the separation of five natural pharmacologically compounds from extracts of rhodiola with satisfactory results. |
| 英文摘要 | a rapid capillary electrophoresis method for the separation of five natural pharmacologically active compounds from extracted rhodiola, namely salidroside, tyrosol, rhodionin, gallic acid and ethyl gallate has been developed. the separation of five natural pharmacologically active compounds was carried out in a fused-silica capillary with 14 mm boric acid, 30 mm sds and 2.5% acetonitrile, adjusted to ph 10.7 with naoh. applied potential was 21 kv. the temperature of the capillary was maintained at 25 degreesc by the instrument thermostating system, with the correlation coefficients of 0.9805-0.9989 for migration time, and relative standards of < 3.52% for peak areas. the established method is rapid and reproducible for the separation of five natural pharmacologically compounds from extracts of rhodiola with satisfactory results. |
| 学科主题 | 生物化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | hydrophilic-interaction chromatography ; micellar electrokinetic chromatography ; separation ; peptides ; phase ; electrochromatography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000225079600017 |
| 公开日期 | 2009-12-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1386]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810008, Peoples R China 2.Qufu Normal Univ, Dept Chem, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Suo, YR,Wang, HL,Li, YL,et al. Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis[J]. chromatographia,2004,60(9-10):589-595. |
| APA | Suo, YR,Wang, HL,Li, YL,You, JM,&Wang, HQ.(2004).Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis.chromatographia,60(9-10),589-595. |
| MLA | Suo, YR,et al."Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis".chromatographia 60.9-10(2004):589-595. |
入库方式： OAI收割
来源：西北高原生物研究所
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