A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences
文献类型：期刊论文
| 作者 | Yang, MH; Zhang, DM; Liu, JQ; Zheng, JH |
| 刊名 | planta medica
 |
| 出版日期 | 2001-11-01 |
| 卷号 | 67期号:8页码:784-786 |
| 关键词 | DNA GENOME |
| 合作状况 | 其它 |
| 中文摘要 | "da-huang" (radix et rhizoma rhei, medicinal rhubarb), a famous and important traditional chinese medicine, has often been confused with the adulterant species in the same genus, rheum. through sequencing the trnl (uaa)/trnf (gaa) regions of chloroplast dna of thirteen species of rheum (three medicinal rhubarb species and ten adulterant ones), a molecular marker of the medicinal species was found. a pair of pcr primers based on the sequences, was thus designed, which amplified a highly specific dna fragment in medicinal rhubarb exclusively, and absent in the adulterants at all under an optimized pcr condition. |
| 英文摘要 | "da-huang" (radix et rhizoma rhei, medicinal rhubarb), a famous and important traditional chinese medicine, has often been confused with the adulterant species in the same genus, rheum. through sequencing the trnl (uaa)/trnf (gaa) regions of chloroplast dna of thirteen species of rheum (three medicinal rhubarb species and ten adulterant ones), a molecular marker of the medicinal species was found. a pair of pcr primers based on the sequences, was thus designed, which amplified a highly specific dna fragment in medicinal rhubarb exclusively, and absent in the adulterants at all under an optimized pcr condition. |
| 学科主题 | 植物学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | plant sciences ; chemistry, medicinal ; pharmacology & pharmacy |
| 研究领域[WOS] | plant sciences ; pharmacology & pharmacy |
| 关键词[WOS] | dna ; genome |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000172234800028 |
| 公开日期 | 2009-12-10 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1423]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Lab Systemat & Evolut Bot, Beijing 100093, Peoples R China 2.Peking Univ, Sch Pharmaceut Sci, Beijing 100871, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, MH,Zhang, DM,Liu, JQ,et al. A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences[J]. planta medica,2001,67(8):784-786. |
| APA | Yang, MH,Zhang, DM,Liu, JQ,&Zheng, JH.(2001).A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences.planta medica,67(8),784-786. |
| MLA | Yang, MH,et al."A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences".planta medica 67.8(2001):784-786. |
入库方式： OAI收割
来源：西北高原生物研究所
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