中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction

文献类型:期刊论文

作者Fang, XH; Liu, JL; Zhang XJ(张雪洁); Wang XF(王晓峰); Li JY(李敬源); Zhang, XJ; Wang, XF; Xu, L; Li, JY
刊名SCIENCE BULLETIN
出版日期2016
卷号61期号:15页码:1187-1194
关键词Cigarette carcinogens Thrombin Thrombomodulin AFM Single-molecule force spectroscopy Molecular simulation
DOI10.1007/s11434-016-1084-0
英文摘要Exposure to cigarette smoke is a major risk factor for cancer and cardiovascular disease. Thrombosis is regarded as the main reason for smoking-related cardiovascular disease. However, the detail mechanism of how smoking promotes thrombosis is not fully understood. In this work, we investigated the impacts of one major cigarette carcinogens 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) as well as its metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) on a key process in thrombosis regulation: thrombin-thrombomodulin (TM) binding. Atomic force microscopy based single-molecule force spectroscopy was applied to measure both in vitro and in vivo binding force of thrombin to TM in the absence and presence of NNK and NNAL respectively. The results revealed that NNK and NNAL can reduce the binding probability of TM and thrombin. The inhibition effect and underlying mechanism was further studied by molecular simulation. As indicated by our results, the cigarette carcinogens could cause a higher risk of thrombosis through the disruption of TM-thrombin interaction.
语种英语
WOS记录号WOS:000381095100008
源URL[http://ir.ihep.ac.cn/handle/311005/260290]  
专题高能物理研究所_多学科研究中心
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Fang, XH,Liu, JL,Zhang XJ,et al. Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction[J]. SCIENCE BULLETIN,2016,61(15):1187-1194.
APA Fang, XH.,Liu, JL.,张雪洁.,王晓峰.,李敬源.,...&Li, JY.(2016).Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction.SCIENCE BULLETIN,61(15),1187-1194.
MLA Fang, XH,et al."Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction".SCIENCE BULLETIN 61.15(2016):1187-1194.

入库方式: OAI收割

来源:高能物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。