Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction
文献类型：期刊论文
| 作者 | Fang, XH; Liu, JL; Zhang XJ(张雪洁); Wang XF(王晓峰) ; Li JY(李敬源); Zhang, XJ; Wang, XF; Xu, L; Li, JY
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| 刊名 | SCIENCE BULLETIN
 |
| 出版日期 | 2016 |
| 卷号 | 61期号:15页码:1187-1194 |
| 关键词 | Cigarette carcinogens Thrombin Thrombomodulin AFM Single-molecule force spectroscopy Molecular simulation |
| DOI | 10.1007/s11434-016-1084-0 |
| 英文摘要 | Exposure to cigarette smoke is a major risk factor for cancer and cardiovascular disease. Thrombosis is regarded as the main reason for smoking-related cardiovascular disease. However, the detail mechanism of how smoking promotes thrombosis is not fully understood. In this work, we investigated the impacts of one major cigarette carcinogens 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) as well as its metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) on a key process in thrombosis regulation: thrombin-thrombomodulin (TM) binding. Atomic force microscopy based single-molecule force spectroscopy was applied to measure both in vitro and in vivo binding force of thrombin to TM in the absence and presence of NNK and NNAL respectively. The results revealed that NNK and NNAL can reduce the binding probability of TM and thrombin. The inhibition effect and underlying mechanism was further studied by molecular simulation. As indicated by our results, the cigarette carcinogens could cause a higher risk of thrombosis through the disruption of TM-thrombin interaction. |
| 语种 | 英语 |
| WOS记录号 | WOS:000381095100008 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/260290]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Fang, XH,Liu, JL,Zhang XJ,et al. Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction[J]. SCIENCE BULLETIN,2016,61(15):1187-1194. |
| APA | Fang, XH.,Liu, JL.,张雪洁.,王晓峰.,李敬源.,...&Li, JY.(2016).Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction.SCIENCE BULLETIN,61(15),1187-1194. |
| MLA | Fang, XH,et al."Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction".SCIENCE BULLETIN 61.15(2016):1187-1194. |
入库方式： OAI收割
来源：高能物理研究所
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