Fullerenol Nanoparticles with Structural Activity Induce Variable Intracellular Actin Filament Morphologies
文献类型：期刊论文
| 作者 | Xing, GM; Jin JJ(金俊江); Dong Y(董莹); Wang Y(王营); Xia L(夏林) ; Gu WH(古伟宏); Bai X(白雪); Chang YN(常亚男); Zhang MY(张铭倚); Chen K(陈奎)
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| 刊名 | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
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| 出版日期 | 2016 |
| 卷号 | 12期号:6页码:1234-1244 |
| 关键词 | Fullerenol Structural Activity Actin Filament Morphologies Cofilin-Fullerenol-Actin Rods |
| DOI | 10.1166/jbn.2016.2251 |
| 通讯作者 | 李娟 ; 赵丽娜 ; 邢更妹 |
| 英文摘要 | Fullerenol nanoparticles are promising for various biological applications; many studies have shown that they induce variable and diverse biological effects including side effects. Separation and purification of two fractions of fullerenols has demonstrated that they have varied chemical structures on the surfaces of their carbon cages. Actin is an important structural protein that is able to transform functional structures under varied physiological conditions. We assessed the abilities of the two fractions of fullerenols to attach to actin and induce variable morphological features in actin filament structures. Specifically the fullerenol fraction with a surface electric charge of -1.913 +/- 0.008q (x 10(-6) C) has percentages of C-OH and C=O on the carbon cage of 16.14 +/- 0.60 and 17.55 +/- 0.69. These features allow it to form intermolecular hydrogen bonds with actin at a stoichiometric ratio of four fullerenols per actin subunit. Molecular simulations revealed these specific binding sites and binding modes in atomic details in the interaction between the active fullerenol and actin filament. Conversely, these interactions were not possible for the other fraction of fullerenol with that percentages of C-OH and C=O on the carbon cage were 15.59 +/- 0.01 and 1.94 +/- 0.11. Neither sample induced appreciable cytotoxicity or acute cell death. After entering cells, active fullerenol binding to actin induces variable morphological features and may transform ATP-actin to ADP-actin. These changes facilitate the binding of ADF/cofilin, allowing cofilin to sever actin filaments to form cofilin/actin/fullerenol rods. Our findings suggest that fullerenol with structural activity binding disturbs actin filament structure, which may inhibit locomotion of cell or induce chronic side effects in to cells. |
| 语种 | 英语 |
| WOS记录号 | WOS:000375968500009 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/260302]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Xing, GM,Jin JJ,Dong Y,et al. Fullerenol Nanoparticles with Structural Activity Induce Variable Intracellular Actin Filament Morphologies[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2016,12(6):1234-1244. |
| APA | Xing, GM.,金俊江.,董莹.,王营.,夏林.,...&Zhao, LN.(2016).Fullerenol Nanoparticles with Structural Activity Induce Variable Intracellular Actin Filament Morphologies.JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,12(6),1234-1244. |
| MLA | Xing, GM,et al."Fullerenol Nanoparticles with Structural Activity Induce Variable Intracellular Actin Filament Morphologies".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 12.6(2016):1234-1244. |
入库方式： OAI收割
来源：高能物理研究所
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