CryoEM structure of yeast cytoplasmic exosome complex
文献类型：期刊论文
| 作者 | Liu, JJ; Niu, CY; Wu, Y; Tan, D; Wang, Y; Ye, MD; Liu, Y; Zhao, WW; Zhou, K; Liu, QS |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2016 |
| 卷号 | 26期号:7页码:822-837 |
| 关键词 | exosome RNA decay Ski7 Rrp6 cryo-EM |
| DOI | 10.1038/cr.2016.56 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The eukaryotic multi-subunit RNA exosome complex plays crucial roles in 3'-to-5' RNA processing and decay. Rrp6 and Ski7 are the major cofactors for the nuclear and cytoplasmic exosomes, respectively. In the cytoplasm, Ski7 helps the exosome to target mRNAs for degradation and turnover via a through-core pathway. However, the interaction between Ski7 and the exosome complex has remained unclear. The transaction of RNA substrates within the exosome is also elusive. In this work, we used single-particle cryo-electron microscopy to solve the structures of the Ski7-exosome complex in RNA-free and RNA-bound forms at resolutions of 4.2 angstrom and 5.8 angstrom, respectively. These structures reveal that the N-terminal domain of Ski7 adopts a structural arrangement and interacts with the exosome in a similar fashion to the C-terminal domain of nuclear Rrp6. Further structural analysis of exosomes with RNA substrates harboring 3' overhangs of different length suggests a switch mechanism of RNA-induced exosome activation in the through-core pathway of RNA processing. |
| WOS关键词 | MESSENGER-RNA DECAY ; MOLECULAR-DYNAMICS ; ELECTRON-MICROSCOPY ; CRYSTAL-STRUCTURE ; QUALITY-CONTROL ; NUCLEAR EXOSOME ; SKI COMPLEX ; DEGRADATION ; EXORIBONUCLEASE ; RECRUITMENT |
| 语种 | 英语 |
| WOS记录号 | WOS:000379495300010 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/260389]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Liu, JJ,Niu, CY,Wu, Y,et al. CryoEM structure of yeast cytoplasmic exosome complex[J]. CELL RESEARCH,2016,26(7):822-837. |
| APA | Liu, JJ.,Niu, CY.,Wu, Y.,Tan, D.,Wang, Y.,...&刘全胜.(2016).CryoEM structure of yeast cytoplasmic exosome complex.CELL RESEARCH,26(7),822-837. |
| MLA | Liu, JJ,et al."CryoEM structure of yeast cytoplasmic exosome complex".CELL RESEARCH 26.7(2016):822-837. |
入库方式： OAI收割
来源：高能物理研究所
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