Evaluation of the toxicity of graphene oxide exposure to the eye
文献类型：期刊论文
| 作者 | Wu, W; Yan L(晏亮) ; Yan, L; Wu, Q; Li, YJ; Li, QY; Chen, SY; Yang, YL; Gu, ZJ; Xu, HW
|
| 刊名 | NANOTOXICOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 10期号:9页码:1329-1340 |
| 关键词 | Environmental toxicology exposure nanotoxicology occupational health toxicology |
| DOI | 10.1080/17435390.2016.1210692 |
| 通讯作者 | 谷战军 |
| 英文摘要 | Graphene and its derivatives are the new carbon nanomaterials with the prospect for great applications in electronics, energy storage, biosensors and medicine. However, little is known about the toxicity of graphene or its derivatives in the case of occasional or repeated ocular exposure. We performed in vitro and in vivo studies to evaluate the toxicity of graphene oxide (GO) exposure to the eye. Primary human corneal epithelium cells (hCorECs) and human conjunctiva epithelium cells (hConECs) were exposed to GO (12.5-100g/mL). Acute GO exposure (2h) did not induce cytotoxicity to hCorECs. However, short-term GO exposure (24h) exerted significant cytotoxicity to hCorECs and hConECs with increased intracellular reactive oxygen species (ROS). Glutathione (GSH) reduced the GO-induced cytotoxicity. We further performed acute eye irritation tests in albino rabbits according to the Organization for Economic Cooperation and Development (OECD) guidelines, and the rabbits did not exhibit corneal opacity, conjunctival redness, abnormality of the iris, or chemosis at any time point after the instillation of 100g/mL of GO. However, 5-day repeated GO exposure (50 and 100g/mL) caused reversible mild corneal opacity, conjunctival redness and corneal epithelium damage to Sprague-Dawley rats, which was also alleviated by GSH. Therefore, our study suggests that GO-induced time- and dose-dependent cytotoxicity to hCorECs and hConECs via oxidative stress. Occasional GO exposure did not cause acute eye irritation; short-term repeated GO exposure generally resulted in reversible damage to the eye via oxidative stress, which may be alleviated by the antioxidant GSH. |
| 语种 | 英语 |
| WOS记录号 | WOS:000383442200012 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/260406]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Wu, W,Yan L,Yan, L,et al. Evaluation of the toxicity of graphene oxide exposure to the eye[J]. NANOTOXICOLOGY,2016,10(9):1329-1340. |
| APA | Wu, W.,晏亮.,Yan, L.,Wu, Q.,Li, YJ.,...&谷战军.(2016).Evaluation of the toxicity of graphene oxide exposure to the eye.NANOTOXICOLOGY,10(9),1329-1340. |
| MLA | Wu, W,et al."Evaluation of the toxicity of graphene oxide exposure to the eye".NANOTOXICOLOGY 10.9(2016):1329-1340. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。