Structural insight into the E. coli HigBA
文献类型：期刊论文
| 作者 | Yang, JS; Zhou K(周柯) ; Liu P(刘鹏); Dong YH(董宇辉); Gao ZQ(高增强); Liu QS(刘全胜); Zhou, K; Liu, P; Dong, YH; Gao, ZQ
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| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 出版日期 | 2016 |
| 卷号 | 478期号:4页码:1521-1527 |
| 关键词 | Toxin-antitoxin HigBA |
| DOI | 10.1016/j.bbrc.2016.08.131 |
| 通讯作者 | 刘全胜 |
| 英文摘要 | The toxin-antitoxin system is ubiquitously existed in bacteria and archaea, performing a wide variety of functions modulating cell fitness in response to environmental cues. In this report, we solved the crystal structure of the toxin-antitoxin HigBA complex from E. coli K-12 to 2.7 angstrom resolution. The crystal structure of the HigBA complex displays a hetero-tetramer (HigBA)(2) form comprised by two HigB and two HigA subunits. Each toxin HigB resumes a microbial RNase T1 fold, characteristic of a three antiparallel beta-sheet core shielded by a few alpha-helices at either side. Each antitoxin HigA composed of all alpha-helices resembles a "C"-shaped clamp nicely encompassing a HigB in the (HigBA)(2) complex. Two HigA monomers dimerize at their N-terminal domain. We showed that HigA helix alpha 1 was essential for HigA dimerization and the hetero-tetramer (HigBA)(2) formation, but not for a hetero-dimeric HigBA formation. HigA dimerization mediated by helix alpha 1 was dispensable for DNA-binding, as a heterodimeric HigBA complex still bound to the higBA operator in vitro. The HigA C-terminal domain with a helix-turn-helix fold was essential for DNA binding. We also defined two palindromes in higBA operator specifically recognized by HigA and HigBA in vitro. (C) 2016 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000384390200006 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/260412]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Yang, JS,Zhou K,Liu P,et al. Structural insight into the E. coli HigBA[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,478(4):1521-1527. |
| APA | Yang, JS.,周柯.,刘鹏.,董宇辉.,高增强.,...&Liu, QS.(2016).Structural insight into the E. coli HigBA.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,478(4),1521-1527. |
| MLA | Yang, JS,et al."Structural insight into the E. coli HigBA".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 478.4(2016):1521-1527. |
入库方式： OAI收割
来源：高能物理研究所
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