Measurement of protein size in concentrated solutions by small angle X-ray scattering
文献类型：期刊论文
| 作者 | Liu, J; Liu J(刘俊) ; Li ZH(李志宏); Wei YR(魏彦茹); Wang WJ(王文佳); Wang B(汪冰); Liang HL(梁红丽); Gao YX(高愈希); Li, ZH; Wei, YR
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| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2016 |
| 卷号 | 25期号:8页码:1385-1389 |
| 关键词 | protein size inter-particle interference distance distribution function small angle X-ray scattering |
| DOI | 10.1002/pro.2957 |
| 通讯作者 | 李志宏 |
| 英文摘要 | By simulations on the distance distribution function (DDF) derived from small angle X-ray scattering (SAXS) theoretical data of a dense monodisperse system, we found a quantitative mathematical correlation between the apparent size of a spherically symmetric (or nearly spherically symmetric) homogenous particle and the concentration of the solution. SAXS experiments on protein solutions of human hemoglobin and horse myoglobin validated the correlation. This gives a new method to determine, from the SAXS DDF, the size of spherically symmetric (or nearly spherically symmetric) particles of a dense monodisperse system, specifically for protein solutions with interference effects. |
| 语种 | 英语 |
| WOS记录号 | WOS:000380068700003 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/260455]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Liu, J,Liu J,Li ZH,et al. Measurement of protein size in concentrated solutions by small angle X-ray scattering[J]. PROTEIN SCIENCE,2016,25(8):1385-1389. |
| APA | Liu, J.,刘俊.,李志宏.,魏彦茹.,王文佳.,...&Gao, YX.(2016).Measurement of protein size in concentrated solutions by small angle X-ray scattering.PROTEIN SCIENCE,25(8),1385-1389. |
| MLA | Liu, J,et al."Measurement of protein size in concentrated solutions by small angle X-ray scattering".PROTEIN SCIENCE 25.8(2016):1385-1389. |
入库方式： OAI收割
来源：高能物理研究所
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