Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis
文献类型：期刊论文
| 作者 | Wang, HF; Wang, M ; Feng, WY; Li, L; Zhang, XY; Shi, JW; Wang M(王萌); Feng WY(丰伟悦); Zhang XY(张欣颖); Shi JW(史俊稳)
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| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
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| 出版日期 | 2016 |
| 卷号 | 43期号:8页码:739-746 |
| 关键词 | ICP-MS single cell analysis metal tag mass cytometry laser ablation |
| DOI | 10.16476/j.pibb.2016.0003 |
| 通讯作者 | 王萌 |
| 英文摘要 | Previous studies generally focused on the cell colony and the obtained information was commonly the average of many cell individuals. However, each cell has a different behavior, which is known as cell heterogeneity. Single cell analysis can accurately obtain valuable information of each cell in the microenvironment and thus there is an urgent need for single cell analysis. Many methods have been successfully employed to single cell analysis, such as flow cytometry, fluorescence microscopy, capillary electrophoresis and microfluidic chips. In these methods, single cells are usually stained by a fluorescent label (e.g. fluorescein, quantum dots, green fluorescent protein, etc.) and then detected via the fluorescent signal. However, simultaneous analysis of multiple parameters in a single cell is always challenging because of the overlap in fluorescent spectrum. In addition, the linear range of the fluorescence method is relatively narrow, making difficulty for accurate quantification, especially when comparing signals with considerable difference. To meet these challenges, a new method based on inductively coupled plasma mass spectrometry (ICP-MS) has emerged for single cell analysis. Intracellular elements can be determined directly by ICP-MS at a single cell level. In combination with labeling techniques(e.g. element labeling of an antibody), biomolecules in single cells can also be determined via elements analysis by ICP-MS. This paper summaries both the ICP-MS-based methodology and selected applications in immunoassay, disease detection, drug screen, and nanoanalysis at a single cell level. A prospective of this method and its applications is also discussed. |
| 语种 | 中文 |
| WOS记录号 | WOS:000381846600002 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/260464]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Wang, HF,Wang, M,Feng, WY,et al. Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2016,43(8):739-746. |
| APA | Wang, HF.,Wang, M.,Feng, WY.,Li, L.,Zhang, XY.,...&Feng WY.(2016).Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,43(8),739-746. |
| MLA | Wang, HF,et al."Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 43.8(2016):739-746. |
入库方式： OAI收割
来源：高能物理研究所
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