Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots
文献类型：期刊论文
| 作者 | Chai ZF(柴之芳) ; Li, YY; Zhao, JT; Li, YF; Xu, XH; Zhang, BW; Liu, YJ; Cui, LW; Li, B; Gao, YX
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| 刊名 | METALLOMICS
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| 出版日期 | 2016 |
| 卷号 | 8期号:7页码:663 |
| DOI | 10.1039/c5mt00264h |
| 通讯作者 | 高愈希 |
| 英文摘要 | The toxicity mechanisms of rice roots under inorganic mercury (IHg) or methylmercury (MeHg) stress were investigated using metalloproteomic approaches. Rice seedlings were cultivated in nutrient solutions with IHg or MeHg for three weeks. Proteins were extracted from the roots and separated by two-dimensional electrophoresis (2-DE). Differentially expressed proteins were analyzed using ESI-MS/MS and identified by PMF. 26 and 29 protein spots were differentially expressed in the IHg-and MeHg-exposed roots, respectively. The proteins responsive to Hg exposure are involved in antioxidative defense, sulfur and glutathione metabolism, carbohydrate and energy metabolism, programmed cell death, and pathogen defense. Chitinase and salt stress-induced proteins exhibited a greater differentially expression in response to MeHg stress compared to IHg stress. Hg-binding proteins were detected by the combined use of 1-DE, SRXRF, and ESI-MS/MS. The results showed that Hg was bound to proteins of 15-25 kDa in rice roots under Hg stress. The Hg contents in the band under IHg stress were remarkably higher than those under MeHg. Hg binds to proteins, which leads to irreversible damage of root growth. Rice roots changed the related protein expression levels in response to Hg stress. These results may provide new insights into the mechanism of toxicity of IHg and MeHg in rice. |
| 语种 | 英语 |
| WOS记录号 | WOS:000379572300005 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/261055]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Chai ZF,Li, YY,Zhao, JT,et al. Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots[J]. METALLOMICS,2016,8(7):663. |
| APA | 柴之芳.,Li, YY.,Zhao, JT.,Li, YF.,Xu, XH.,...&高愈希.(2016).Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots.METALLOMICS,8(7),663. |
| MLA | 柴之芳,et al."Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots".METALLOMICS 8.7(2016):663. |
入库方式： OAI收割
来源：高能物理研究所
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