A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)
文献类型：期刊论文
| 作者 | Zhang, JC; Chen, J; Zhang, HT; Hu Y(胡毅); Chai ZF(柴之芳) ; Zhao YL(赵宇亮); Chen J(陈俊); Lu, HR; Lu HR(卢会茹); Hu, Y
|
| 刊名 | TALANTA
 |
| 出版日期 | 2016 |
| 卷号 | 146页码:477 |
| 关键词 | Copper Cysteine Fluorescent probe Ellman's reagent Cell imaging |
| DOI | 10.1016/j.talanta.2015.09.014 |
| 通讯作者 | 胡毅 |
| 英文摘要 | In this study, Cu(II)-mediated differential alteration of cysteine (Cys) reactivity is reported by using a Cys-specific fluorescent probe. The probe could react with Cys to give out strong fluorescence. When Cys was preincubated with Cu(II), the fluorescence of the probe was decreased due to the inhibition of Cys's reactivity by Cu(II). Remarkably, experimental results reveal that the probe could detect Cu(II) at subnanomolar concentrations. In contrast, Cu(II) could only partially inhibit the reaction between Cys and Ellman's reagent (DTNB). Furthermore, selectivity experiments show that Cu(II) is a much more potent inhibitor for Cys compared to other metal ions. Cell imaging experiments also confirm the inhibitory effects of Cu(II) on Cys's reactivity in living cells. We envision that the probe could add a useful tool for sensitive and selective detection of Cu(II) for biomedical research. (C) 2015 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000363815600068 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/261058]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, JC,Chen, J,Zhang, HT,et al. A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)[J]. TALANTA,2016,146:477. |
| APA | Zhang, JC.,Chen, J.,Zhang, HT.,胡毅.,柴之芳.,...&Liu, RC.(2016).A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II).TALANTA,146,477. |
| MLA | Zhang, JC,et al."A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)".TALANTA 146(2016):477. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。