Interactions between uranium(VI) and phosphopeptide: experimental and theoretical investigations
文献类型：期刊论文
| 作者 | Wu, QY ; Zhai, FW; Liu, Y; Yuan, LY; Chai, ZF; Shi, WQ; Liu Y(刘洋); Yuan LY(袁立永); Chai ZF(柴之芳); Shi WQ(石伟群)
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| 刊名 | DALTON TRANSACTIONS
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| 出版日期 | 2016 |
| 卷号 | 45期号:38页码:14988 |
| DOI | 10.1039/c6dt03009b |
| 英文摘要 | Uranium is an essential actinide element in nuclear fuel cycles, and protein phosphorylation is one type of most important post-translational modifications. It is of great interest to study the interactions between uranyl ions and phosphorylated proteins. In this study, a phosphorylated pentapeptide (WpTPpTW, P-1) motif was designed as a model to mimic possible coordination sites in genuine phosphorylated proteins. Electrospray ionization mass spectrometry (ESI-MS) results suggested that uranyl-P-1 complexes with chemical stoichiometry of 1 : 1 and 1 : 2 were both available. The conditional stability constant of the 1 : 1 complex uranyl-P-1 was determined to be 6.6 +/- 0.2 at pH 4.0 by tryptophan fluorescence titrations, which is almost three orders of magnitude higher than that of the complex of nonphosphorylated peptide. The results of extended X-ray absorption fine structure (EXAFS) combined with density functional theoretical calculations suggested that uranyl ions coordinated with one phosphoryl and carboxyl groups of P-1 in a mono-dentate fashion, and three water molecules. This study on the simple metal-peptide system could provide basic information for locating the uranyl coordination site in some important phosphorylated proteins which is useful for evaluating the chemical toxicity of uranyl in vivo. |
| 语种 | 英语 |
| WOS记录号 | WOS:000385198400017 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/261093]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_加速器中心 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Wu, QY,Zhai, FW,Liu, Y,et al. Interactions between uranium(VI) and phosphopeptide: experimental and theoretical investigations[J]. DALTON TRANSACTIONS,2016,45(38):14988. |
| APA | Wu, QY.,Zhai, FW.,Liu, Y.,Yuan, LY.,Chai, ZF.,...&吴群燕.(2016).Interactions between uranium(VI) and phosphopeptide: experimental and theoretical investigations.DALTON TRANSACTIONS,45(38),14988. |
| MLA | Wu, QY,et al."Interactions between uranium(VI) and phosphopeptide: experimental and theoretical investigations".DALTON TRANSACTIONS 45.38(2016):14988. |
入库方式： OAI收割
来源：高能物理研究所
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