molecular cloning and expression analysis of ch-penaeidin, an antimicrobial peptide from chinese shrimp, fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Kang CH ; Wang JX ; Zhao Xianfeng ; Yang XM ; Shao HL ; Xiang JH |
| 刊名 | Fish & Shellfish Immunology
 |
| 出版日期 | 2004 |
| 卷号 | 16期号:4页码:513-525 |
| 关键词 | Fenneropenaeus chinensis penaeidin antimicrobial peptides gene expression innate immunity ANTIBACTERIAL PEPTIDE LITOPENAEUS-VANNAMEI PENAEUS-VANNAMEI INNATE IMMUNITY HEMOCYTES CRAB GRANULOCYTES TACHYPLESIN CHALLENGE DECAPODA |
| 通讯作者 | Wang, JX (通讯作者), Shandong Univ, Sch Life Sci, Jinan 250100, Shandong Peoples R China |
| 收录类别 | SCI |
| 公开日期 | 2010-08-25 |
| 附注 | A new member of antimicrobial peptide genes of the penaeidin family, Ch-penaeidin, has been cloned from the haemocytes of Chinese shrimp, Fenneropenaeus chinensis, by reverse transcription PCR (RT-PCR), 3'-rapid amplification of cDNA end (3'-RACE) and smart cDNA methods. The Ch-penaeidin cDNA was 655 bp and the open reading frame of the cDNA encoded a 71 amino acid peptide. Ch-penaeidin contained a putative NH2-terminal signal Sequence (1-19) followed by a mature peptide (20-71). The sequence identify with other penaeidins from Litopenaeus vannamei and Litopenaeus setiferus is between 48% and 71%. The signal sequence of Ch-penaeidin is almost completely identical to that of other penaeidins, while differing relatively in the N-terminal domain of the mature peptide. Ch-penaeidin was designated as a novel member of class penaeidin 3 according to phylogenetic analysis. The Mature peptide. with a predicted molecular weight of 5589.32 Da, and a pI of 9.77, has eight positively charged amino acids and no negatively charged amino acids. The expression and distribution of Ch-penaeidin in Unchallenged shrimps were studied by RT-PCR, Northern blot and in situ hybridisation. The results showed that the Ch-penaeidin transcripts were detected in haemocytes (granular haemocytes), heart, gill, intestine, and subcuticular epithelia of the shrimp. and that Ch-penaeidin was constitutively expressed mainly in haemocytes. (C) 2003 Elsevier Ltd. All rights reserved. |
| 源URL | [http://124.16.136.157/handle/311060/4972]  |
| 专题 | 软件研究所_信息安全国家重点实验室_期刊论文 |
| 推荐引用方式 GB/T 7714 | Kang CH,Wang JX,Zhao Xianfeng,et al. molecular cloning and expression analysis of ch-penaeidin, an antimicrobial peptide from chinese shrimp, fenneropenaeus chinensis[J]. Fish & Shellfish Immunology,2004,16(4):513-525. |
| APA | Kang CH,Wang JX,Zhao Xianfeng,Yang XM,Shao HL,&Xiang JH.(2004).molecular cloning and expression analysis of ch-penaeidin, an antimicrobial peptide from chinese shrimp, fenneropenaeus chinensis.Fish & Shellfish Immunology,16(4),513-525. |
| MLA | Kang CH,et al."molecular cloning and expression analysis of ch-penaeidin, an antimicrobial peptide from chinese shrimp, fenneropenaeus chinensis".Fish & Shellfish Immunology 16.4(2004):513-525. |
入库方式： OAI收割
来源：软件研究所
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